Fluorescence Spectroscopy

Question 1

What is fluorescent spectroscopy?

Answer 1

It is the measurement of radiation emission from an analyte as it relaxes down the ground state.

Question 2

What energy difference is expected between absorption and emission in molecules?

Answer 2

The absorption will have a higher optimal wavelength, whereas the emission energy will be emitted as a lower energy wavelength (Longer wavelength).

Question 3

What is the process of fluorescent spectroscopy?

Answer 3

A monochromator will allow a particular wavelength through to excite our analyte whilst also being reflected to a detector to measure incident intensity. a monochromater is then used to filter a particular wavelength that is being emitted from the now excited analyte. This emission is measured and compared against incident light.

Question 4

Why is the light measure at 90 degree angles to the incident light?

Answer 4

This prevents scattering of unwanted light sources and saturating of the detector due to the lamp.