Flow cytometry, CBC and ELISA

Question 1

What is flow cytometry

Answer 1

Measurement of cells charcteristics in a liquid suspension

Question 2

What is cytometry?

Answer 2

The measurement of cell characteristics such as shape, morphology, size , intracellular complexity( which effects the light going through)

Question 3

What are the scatters in flow cytometry and what do they measure?

Answer 3

• The size of the cells is measured by the forward scatter

-For the granularity (the density) the side scatter is measured because dense cells deflect the laser to the side

Question 4

When forward and side scatters are combined what is the order of the cells in the image?

Answer 4

• Big part Is the granulocytes made from neutrophils
• Middle is monocytes- less density
• Last at the bottom - lymphocytes no granularity with dead cells hat give signal

Question 5

What does CD8 and CD4 on flow cytometry ?

Answer 5

CD8 signal is a smear
CD4 signal is compact

Question 6

What is CBC used for ?

Answer 6

-Measuring the RBC and WBCs

• Ideal for babies as not too much blood is needed

-Can be used for Anaemia (RBC count)
-Infection(lymphocytes Covid 19)
-Inflammation (allergy eosinophils)
-Leukaemia
-Bleeding (platelets)