Flow Cytometry Flashcards
Divine fluid or liquid window to the body’s inner workings
Urine
Urinalysis began ________
6000 years ago
What was urinalysis called 6000 years ago?
Uroscopy
Described bubbles on the surface of urine as an indication of kidney disease
Hippocrates
Bubbles in urine
Proteinuria
Collection of urine sample
Mid stream clean catch
How do you mix urine sample?
Gently invert
Volume of urine transferred into test tube
10 mL
Proper speed and time of centri for urine
400 relative centrifugal force for 5 minutes
Proper decanting of supernatant
Aspirate
Volume for slide prep of urine
0.2 mL
Minimum number of fields for microscopy
10/lpf or hpf
Kamukhang kamukha ng RBC
Yeast cells
Analysis of the physiologic and chemical properties of cells and other biological particles
Cytometry
Sysmex dye for urinalysis
Polymethine dye (fluorescent dye; stains nucleic acid)
Principle ng sysmex
Hydrofocusing
Size: S-L
NO FLUORESCENCE
Crystals
Size: S-M
Low fluorescence
RBC
Very low size
Low low fluorescence
Bacteria
Small size
Medium fluorescence
Sperm
Small size
Low to medium fluorescence
Yeast like cells
Largest in size
Highest fluorescence
WBC
Small to big size
No fluorescence
Debris
2 graphics used
Scattergram
Histogram
Positive dipstick not only i dicates presence of RBC but also ___ and ____
Hgb and myoglobin
Red or redbrown and clear urine is due to
Hgb and myoglobin
3 aspects of urinalysis in the lab
Macroscopic
Chemical
Microscopic
Fully automated urine analyzer
UX-2000
Name of speaker
Zykhar Batulan
