Flow cytometry

Question 1

Flow cytometry goal

Answer 1

Measures cell types individually, suspended in a fluid using lasers and detectors. Used to monitor how a disease is progressing by measuring cell population

Question 2

Hydrodynamic focusing

Answer 2

Ce;;s moving in one line can be analysed

Question 3

Laser role

Answer 3

Creates light that scatters off cell which can be read

Question 4

What is the liquid that cells are in in FC?

Answer 4

Sheath fluid

Question 5

FSC

Answer 5

Determines cell type in the immune system

Question 6

SSC

Answer 6

Provides information about internal cells

Question 7

Electronics

Answer 7

Transmits the light reflected into an electrical impulse recorded by software

Question 8

Optics

Answer 8

illuminates the cell and filters to direct light to the appropriate receptors

Question 9

Specteral overlap

Answer 9

Light from 2 fluorochromes being detected by multiple detectors

Question 10

Gating

Answer 10

Selecting a specific cell population

Question 11

Name the 3 types of test to monitor the stage of HIV

Answer 11

CD4 count - Healthy individual 500 - 1000, HIV less than 200

Viral load - Measures the amount of virus in the blood

Drug resistance - Determines if specific strain of HIV is resistant to certain anti-HIV medications

Question 12

How HIV infects cells

Answer 12

Enters CD4 cells, males copies of itself and kills CD4 cell, repeats this process, IS must make more cells to counter this but must be faster

Aids sufferers more prone due to less CD4 count, weakens IS