Fixatives Chromates

Question 1

Preserves carbohydrates precipitates all protein

Answer 1

CHROMIC ACID 1-2%

Question 2

● For chromatin, mitochondria, mitotic figures, golgi bodies, RBC, and colloid containing tissues
● DADV: prolonged fixation may lead to blackening of tissue pigment
→ Remedy: wash in running tap H20 before hydration

Answer 2

REGAUD’S/MULLER’S FLUID

Question 3

Study of early degenerative processes and necrosis; Rickettsia and other bacteria

Answer 3

ORTH’S FLUID

Question 4

● Preserves lipids and mitochondria at H 4.5-5.2; cytoplasm, chromatin, and chromosome are fixed
● Corrosive, thus avoid skin contact

Answer 4

POTASSIUM DICHROMATE

Question 5

● Preserves acid mucopolysaccharide
● DADV: prolonged standing->formation of INSOLUBLE LEAD CARBONATE
→ Remedy: add drops of acetic acid to dissolve residue

Answer 5

LEAD