FIXATION

Question 1

PRESERVES TISSUE FROM DECAY

Answer 1

FIXATION

Question 2

It is the most critical step in Histotechnology

Answer 2

Fixation

Question 3

1st goal of Fixation

Answer 3

To preserve the morphologic and chemical integrity of the cell.

Question 4

2nd goal of Fixation

Answer 4

To harden and protect the tissue from the trauma of further handling.

Question 5

Cause of ice crystals artefacts

Answer 5

Slow freezing

Question 6

Tissue should not be more than _______

Answer 6

5 mm.

Question 7

All tissue specimen must be properly ________ and _________.

Answer 7

Labeled and Identified

Question 8

What is the proper amount of volume of the fixative for the tissue specimen.

Answer 8

20-50 times of volume

Question 9

Cause of excessive hardening or brittleness of tissue?

Answer 9

Period of fixation has exceeded

Question 10

Autopsy materials should be fixed asap to prevent ____________ and ____________.

Answer 10

Decomposition and Autolysis

Question 11

Purpose of Dehydration

Answer 11

To avoid excessive distortion of the tissue.

Question 12

Benefit of Water Bath

Answer 12

It removes wrinkles

Question 13

Suitable additives for Mounting

Answer 13

Albumin and Poly-L-lysine