FINALS Flashcards
The technique of growing and maintaining plant cells
Plant Tissue Culture
A type of tissue culture where explants are derived from in-vitro derived plants introduced to a new artificial environment. Commonly used in orchids
Seed Culture
This type of tissue culture involves the isolation of the embryo.
Embryo Culture
Involves the isolation of an organ for in-vitro growth.
Organ Culture
This refers to the unspecialized, unorganized, and a dividing mass of cells.
Callus
A type of tissue culture that involves an unspecialized, unorganized, dividing mass of cells.
Callus culture
As a review, what are the major steps of tissue culture?
- Initiation Phase
- Multiplication Phase
- Root Formation
It is the first phase of tissue culture where the tissue of interest is introduced and sterilized.
Initiation Phase
It is the second step in tissue culture where in-vitro plant material is re-divided and introduced into the medium.
Multiplication Phase
It is the last step in tissue culture where roots are formed.
Root Formation
What are the processes of tissue culture?
- Medium Preparation
- Stock Solution Preparation
- Plant Preparation
Crop improvement entails genetically manipulating plants in a predetermined way, which often involves the transfer of genes from one source or genetic background to another.
Concept of Gene Transfer
What are the 2 methods of emasculation?
- Direct Emasculation
- Indirect Emasculation
A type of emasculation which involves the technique of removing anthers from the flower
Direct Emasculation
A type of emasculation where the anthers are incapacitated without removing them from the flower
Indirect Emasculation
Genetically divergent parents are crossed for recombination of desirable genes.
Divergent Crossing
A type of divergent cross which involves two elite lines that possess adequate traits.
Single Cross
This type of divergent cross involves the multiple crossing to obtain recombinants that consist of desirable traits.
Three-way cross
This method of divergent crossing entails making a large number of crosses.
Di-allele cross
These are conservative ways of crossing plants, because it does not create new traits, but instead combines all present traits in the gene pool.
Convergent Crossing
What commercial gametocides are used in indirect emasculation?
Sodium methylarsenate
What are the types of Indirect emasculation?
- Thermal Inactivation
- Alcohol Emasculation
- Commercial Gametocides
This external reproductive barrier is caused by the geographical distances between two species.
Spatial Isolation Mechanisms
This external reproductive barrier prevents the union of gametes such as mechanical isolation (herkogamy).
Pre-fertilization Reproductive Barriers