Final Test Flashcards
Golgi Stain
A histological stain that fills a small proportion of neurons with a dark, silver-based precipitate
Nissl stain
A histological stain that outlines all cell bodies because the dyes are attracted to RNA, which encircles the nucleus
Autoradiography
A histological technique that shows the distribution of radioactive chemicals in tissues
Immunocytochemistry (ICC)
A method for detecting a particular protein in tissues in which (1) an antibody recognizes and binds to the protein and (2) chemical methods are then used to leave a visible reaction product around each antibody
In situ hybridization
A method for detecting particular RNA transcripts in tissue sections by providing a nucleotide probe that is complementary to, and will therefore hybridize with, the transcript of interest
immediate early genes (IEGs)
A class of genes that show rapid but transient increases in expression in cells that have become activated
c-fos
An immediate early gene commonly used to identify activated neurons
horseradish peroxidase (HRP)
An enzyme found in horseradish and other plants that is used to determine the cells of origin in a particular set of axons