Final Study Guide Flashcards
What are the two nucleic acids?
DNA
RNA
What are the components of a nucleic acid?
Polymers of nucleotides
What are genes?
Regions of DNA that encode a functional RNA or protein
What is the function of DNA?
Storing genetic code
What are the components of a nucleotide?
Nitrgenous base, Pentose Sugar, Phosphate,
What are the two types of nucleotides?
Purines (Larger)
Pyrimidines (Smaller)
What are the 5 nucleotides - which are purines and which are pyrimidines?
Purines - 2 ring:
- Adenine
- Guanine
Pyrimidines - 1 ring:
- Thymine (DNA)
- Uracil (RNA)
- Cytosine
What is a pentose sugar?
What are the two pentose sugars in nucleotides?
Which one is in RNA? DNA?
5 Carbon Sugar
RNA - Ribose
DNA - Dioxyribose
Which nucleotide is only used in DNA? RNA?
DNA - Thyamine
RNA - Uracil
How do the nucleotides pair up?
Adenine - Thymine / Uracile Guanine - Cytosine
(Apples in the Tree or Under, Cars in the Garage)
What holds the two strands of DNA together?
“Zippered” with HYDROGEN BONDS
What are histones? What is chromatin, euchromatin, heterochromatin? What are chromosomes? When are chromosomes present in a cell?
- Histones: Protein that the DNA is wrapped around
- Chromatin: Highly compacted nucleoprotein structure
- Euchromatin: Active chromatin that gets transcribed regularly
- Heterochromatin: Conserved chromatin
- Chromosomes: HIGHLY compacted X shaped chromatin structures - visible only during cell division
DNA/RNA - How many strands does each have?
RNA is SINGLE stranged (doubles back on itself to pair)
DNA is a double stranded in a helix
DNA/RNA - Which pentose sugar is found in each?
RNA - Ribose
DNA - Deoxyribose
DNA/RNA - Which nucleic acid has multiple forms?
RNA
DNA/RNA - Which nucleotide is unique to each?
RNA - Uracil, DNA - Thymine
DNA/RNA - Which may have enzymatic activity?
RNA - these are known as ribozymes
What are the three types of RNA we studied? What is the function of each?
- mRNA - messenger RNA, blueprint for proteins
- rRNA - Forms structural component of Ribosomes (w/ proteins)
- tRNA - Worker molecules that bring amino acids to ribosome for protein synthesis
What is the Central Dogma of Molecular Biology? What is the order of the flow of biological information?
The transfer of sequential information —
DNA -> RNA -> Protein
What is the enzyme that synthesizes daughter DNA strands in the process of DNA replication?
DNA Polymerase
What is transcription? What enzyme performs transcription?
Reading the DNA nucleotides and assembling a new piece of RNA based on the instruction in the DNA (Happens in the NUCLEUS), and by RNA Polymerase
What is translation? What structure performs translation?
Turning the RNA into a protein, leaves the nucleus and goes to the Ribosomes which assembles the protein
What is tRNA? What is its function?
tRNA is transfer RNA, carries amino acid and matches its anticodon to the mRNA codon to assemble the protein
Know the structure of chromosomes: chromatids, centromere, telomere, etc.
- Chromosome: Two strands of chromatin called chromatids, highly compacted
- Centromere: connects them
- Telomere: End cap
How many pairs of chromosomes do humans have? What is an autosome? What is a sex chromosome? What are the sex chromosomes in humans?
- Humans have 23 pairs of Chromosomes
- Autosome is 1-22
- Final 23rd pair is the sex chromosome *XY or XX*
What is a karyotype?
A picture of chromosomes during cellular division that measures the shape and size of the chromosomes
What is a mutation? What are the different types of mutations?
Mutation is a change in the DNA sequence of an organism.
- Point Mutation
- Insertions
- Deletions
- Chromosomal abnormalities
What are the three types of point mutations?
- Missense mutation - causes wrong aminal acid to be inserted
- Silent mutation - point mutation that doesn’t affect the amino acid
- Nonsense mutation - causes a stop codon to be inserted prematurely causing the premature cessation of protein translation
What is an insertion mutation? A deletion mutation? What is a frameshift mutation?
- Insertion: One or more nucleotides is inserted
- Deletion: removal of one or more nucleotides
- Frameshift: Both insertions and deletions can cause frameshift - which alters the DNA product
Know the processes of binary fission, mitosis, and meiosis. What type of cells perform each? Are the daughter cells unique or identical to the parent cell?
Binary Fission - Used by Prokaryotic cells, asexual reproduction, single loop of DNA replicates, membrane forms, cell divides into 2 genetically identical organisms
Mitosis - Process of eukaryotic cell division, genetically identical daugher cells
Meiosis - Eukaryotic cells to reproduce daughter cells with half of the gentic information, sexual reproduction, genetically unique individual
What is asexual reproduction? Sexual reproduction?
- Asexual - only one parent cell necessary (utilized by single celled organisms)
- Sexual - combining of two parents to create a gentically unique individual
What is the cell cycle? What are the phases of the cell cycle? Which phase is longest?
All the stages of growth and division for a eukaryotic cell
Interphase, Mitosis and Cytokinesis
Interphase is the longest
What are the stages of interphase? In which phase is DNA replicated?
- G1 - First gap phase - cytoplasmic increase (G0 if it never divides again)
- S Phase, Synthesis/DNA replication
- G2 - Second gap phase - Cytoplasmic growth
What are sister chromatids?
2 identical copies of a chromosome (after replication) attached at a centromere
What are the phases of mitosis?
What are the hallmark events associated with each phase?
- Prophase - Chromosomes condense, form spindle, nucleolus disappears, nuclear membrane disassembles
- Metaphase - Chromosomes align at the Middle
- Anaphase - Sister Chromatids break at centromere and move toward opposite (cytokinesis begins)
- Telophase - spindle fibers disassemble, nuclear membrane reforms, chromosomes uncoil, nucleolus reforms (cytokinesis complete - cleaved!)
What is cytokinesis?
Cell splitting at the end of Mitosis into two daughter cells
What are determination and differentiation?
Determination - proccess of which genes will express when the cell is mature
Differentiation - when the cell becomes a specific type of cell
What are the phases of meiosis?
Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase II
What is meant by the terms “haploid” and “diploid”?
Which cells are haploid in humans?
Haploid has one copy of genetic information
Diploid has 2 copies
Only sex cells created in the process of meiosis are haploid
What are gonads? What are gametes?
Gonads are the sex organs where meisosis occurs - Testes in males, Ovaries in females
Gamete is a reproductive cell - eggs, sperm
Fertilization joins two haploid cells to create a diploid cell that is genetically unique
What are synapsis and crossing over? Why are they important?
When homologous pairs pair up and exhange limbs of genetic information to create mixed up DNA - this is what allows for genetic variation!
What are homologous pairs?
2 Chromosomes with the same genes, one from mother and one from the father
Know the definitions of the following terms: genetics, gene, offspring, hybrid, character(istic), heritable, trait, allele, genome.
- Genetics: Branch of science that studies how the characteristics of living organisms are inherited
- Gene: Piece of DNA that codes for a protein
- Offspring: descendants of a set of parents
- Hybrid: Offspring of genetically different parents
- Character(istic): a feature of an organism (i.e. eye color)
- Heritable: character trait passed from parent to offspring
- Trait: particular form of a character (i.e. blue eye) Allele: specific version of a gene
- Genome: set of al the genes necessary for an organisms characteristics
What is an organisms genotype? Its phenotype?
- Genotype: The combination of alleles that are present in the organisms cells
- Phenotype: The resulting outwardly apearance