Final Review Flashcards
A McFarland standard of 0.5 corresponds to a bacterial count of: a. 1.5x10(6) b. 1.0x10(8) c. 1.5x10(8) d. 1.0x10(4)
c. 1.5x10(8)
The mode of action of erythromycin and azithromycin is: a. inhibition of protein synthesis b. inhibition of nucleic acid c. inhibition of cell wall d. alters the cell membrane
a. inhibition of protein synthesis
Quality control records for media and reagents should be maintained a minimum of: a. one year b. three years c. two years d. five years
c. two years
True or False: It is not necessary to perform quality control on user prepared MacConkey plates because it is exempt.
False
The spectrum of activity for aminoglycosides is: a. gram positive aerobic bacteria b. gram negative aerobic bacteria c. gram negative anaerobic bacteria d. aerobic gram positive and gram negative bacteria e. all of the above
d. aerobic gram positive and gram negative bacteria
True of False: Proficiency testing in the microbiology lab will evaluate the accuracy of personnel and procedures
True
Supplemental testing of Enterococcus spp. for detection of high level resistance to which class of antimicrobials would demonstrate if synergy with ampicillin is possible? a. glycopeptides b. fluroquinolones c. macrolides d. aminoglycosides
d. aminoglycosides
All of the following antimicrobials are usually effective against gram negative bacterial except: a. imipenem b. levofloxacin c. vancomycin d. gentamicin
c. vancomycin
Specimens for bacterial culture were submitted from a burn patient. The culture grew a non lactose fermenting gram negative rod identified as P. aeruginosa. Disk diffusion testing was performed with the following results. Ampicillin=16mm Cefazolin=16mm Levofloxacin=23mm Ticarcillin/Clavulanic Acid=18mm Use these CLSI interpretations to determine which category is indicated. LVX is _____ AM is _____ CZ is _____ TIM is _____
LVX - Susceptible AM - Resistant CZ - Intermediate TIM - Intermediate
Growth of Enterococcus faecalis on a vancomyin agar screen plate would indicate that the organism is a. susceptible to vancomycin b. A beta-lactamase producer c. resistant to vancomycin d. resistant to all beta-lactamase
c. resistant to vancomycin
A reference lab has performed a rare manual macrodilution MIC test. Based on the growth in these tubes, what is the MIC value of the antimicrobial? a. 8.0 mcg/ml c. 16.0 mcg/ml d. 8.0 mcg/ml e. 4.0 mcg/ml
d. 8.0 mcg/ml
In order to determine the MBC in the previous question you would: a. Subculture tubes 8.0-256 b. Subculture tubes 0.5-4.0 c. Subculture all tubes d. Interpret the MBC as 8.0 mcg/ml e. Interpret the MBC as 16.0 mcg/ml
a. Subculture tubes 8.0-256
An isolate of E. coli was found to be ESBL positive, as a result it should be reported as resistant to: a. aminoglycosides b. vancomycin c. levofloxacin d. cephalosporins
d. cephalosporins
The use of ATCC reference organisms when performing quality control on established procedures will confirm the following: a. exempt and non exempt b. validity and reliability c. preliminary vs. final results d. proper collection and transport
b. validity and reliability
This test uses the patient’s own serum and can be used to monitor antimicrobic therapy as well as the effects of antibodies and complement. a. disk diffusion b. SBC (SBT) c. MIC (MBC) d. beta-lactamase
b. SBC (SBT)
Providing detailed instruction to nurses for proper specimen collection will effect this area of quality assessment: a. postanalytical b. analytical c. continuous daily monitoring d. preanalytical e. proanalytical
d. preanalytical
True or False: A large disk diffusion zone size will correspond to a low MIC value
True
The action of this antimicrobial in the class of Fluoroquinolones is generally bacteriocidal: a. levofloxacin b. vancomycin c. cephazolin d. erythromycin
a. levofloxacin
When performing routine AST quality control, after the initial 20 day testing period, all labs are required to test their systems: a. daily b. monthly c. weekly d. only when new lot numbers are received
c. weekly
Reference bacterial organisms are available through which of the following sources? a. CLIA b. ATCC c. CLSI d. TQM
b. ATCC
Antimicrobial resistance resulting from the normal genetic, structural, or physiologic state of a microorganism is defined as: a. antagonistic b. bacteriocidal c. acquired d. intrinsic
d. intrinsic
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Which of the following will define test performance, reagent preparation, quality control and results reporting as well as display signature by the microbiology supervisor? a. patient reports b. Q-Probes c. Benchmarks d. standard operating procedure manual
d. standard operating procedure manual
Which bacterial combinations represent the most appropriate quality control organisms when testing a new lot number of catalase test reagent. a. Strep agalactiae and Strep pyogenes b. Staph aureus and Strep pyogenes c. Staph aureus and Staph saprophyticus d. Staph aureus and Staph epi
b. Staph aureus and Strep pyogenes
The testing method demonstrated here will confirm an organism’s resistance to: a. pencillins b. carbapenams c. glycopeptides d. aminogylcosides
b. carbapenams
The MIC value of Oxacillin (OX) when read from this microdilution plate is: a. >4.0 mcg/ml b. 4.0 mcg/ml c. 2.0 mcg/ml d. .5 mcg/ml
a. >4.0 mcg/ml
Which of the following is not an example of intrinsic antibiotic resistance? a. klebsiella va. ampicillin b. staph vs. oxacillin c. E. coli vs. vancomycin d. Pseudomonas vs. trimethorprim and sulfonamides
b. staph vs. oxacillin
True or False: A Mueller Hinton Agar with a depth of 3.5mm will result in zone sizes that are falsely resistant.
False
In recording the results of a microdilution tray, you notice a slight haze is present in all the trimethoprim-sulfa wells. You would record the result as: a. resistant b. susceptible c. intermediate d. invalid
b. susceptible
What is the interpretation for Ampicillin (AM) based on following MIC interpretive standards: >=1.0 mcg/ml: susceptible 2-4 mcg/ml: intermediate >=8 mcg/ml: resistant a. susceptible b. intermediate c. resistant d. unable to interpret
a. susceptible
Preanalytical instructions to patient providers in clinics should indicate that which specimen may be refrigerated? a. sputum b. urine c. CSF d. urogenital swab
b. urine
When performing disk diffusion QC, only one disk out of twelve is out of range. What is the most likely explanation? a. inoculum is too light b. bad disk c. inoculum is too heavy d. mixed culture
b. bad disk
True or False: Panic values should be called immediately to the first available health care worker.
False
True or False: Quality assurance is associated with those external activities that ensure positive patient outcomes
True
Microbiology turnaround time can be defined as the elapsed time between: a. specimen receipt in the lab and reporting of final results b. specimen collection and reporting of final results c. specimen collection and reporting of preliminary gram stain results d. specimen receipt in the lab and initial culture set up
a. specimen receipt in the lab and reporting of final results
3 appropriate organisms to use when performing QC on a new lot of API 20E test systems would be: a. Enterococcus sp., E. coli and Staphylococcus sp b. Klebsiella sp., Haemophilus sp. and Enterococcus sp c. Pseudomonas sp., E. coli, and Neisseria spp d. E. coli, Klebsiella pneumoniae, Proteus mirabilis
d. E. coli, Klebsiella, Proteus mirabilis
True or False: Daily review by the microbiology supervisor of AST results that have been reported is an analytic function
False
True or False: An isolate of Staph aureus is determined to be resistant to Linezolid by MIC method. This antimicrobial (Linezolid) is the most appropriate for treatment.
False
Disk diffusion testing was performed on an isolate of P. aeruginosa from a urine culture. Numerous colonies were observed within the zone of this antimicrobial. What is the most probable cause? a. media was too thick b. media was too thin c. inoculum too heavy d. resistant mutants
d. resistant mutants
Most antimicrobials are cleared by which organ? a. kidney b. pancreas c. lymphatics d. liver
d. liver
True or False: Commercially prepared media are always exempt from quality control testing.
False
Penicillin susceptibility in isolates of Streptococcus pneumoniae can be determined using the following disk diffusion: a. penicillin b. methicilin c. oxacillin d. ampicillin
c. oxacillin
Which of these criteria is not used by the laboratory in determining if antimicrobial susceptibility testing will be performed: a. drugs of choice b. clinical significance c. status of patient’s immune system d. reliable standardized methods for testing
c. status of the patient’s immune system
Quality control for AST disk diffusion was performed and it was noted that numerous zone sizes were too small. The test was repeated with a new lot number of mueller hinton agar and all zone sizes were within an acceptable range. Which is the best explanation for the unacceptable zones? a. media was too thick b. media was too thin c. inoculum too light d. resistant mutants
a. media was too thick
Resistance of Staphylococus to methicillin, penicillin and other beta lactams may be detected by testing with this predictor drug: a. methicillin b. cefazolin c. oxacillin d. vancomycin
c. oxacillin
Protein synthesis is the principal mode of action of which of these antimicrobial pairs. a. erythromycin and ampicillin b. gentamycin and vancomycin c. gentamycin and erythromycin d. vancomycin and levofloxacin
c. gentamycin and erythromycin
Direct beta-lactamase testing may be performed by using a/an: a. cephalosporin (cefinase) disk b. oxacillin c. etest d. disk diffusion
c. etest
The zone of inhibition of disk A, is most likely to correspond to which of the following MIC results. a. 64mcg/ml b. 2.0 mcg/ml c. 1.0 mcg/ml d. <0.5 mcg/ml
a. 64 mcg/ml
Indicate only the criteria that are a part of the CLSI guidelines for AST. (This is an all or nothing question) a. length of incubation for testing b. medium type c. number of antimicrobials to be reported d. McFarland Standard (inoculum) e. age range of patient with infection f. concentration of antimicrobial begin tested
a. length of incubation for testing b. medium type d. McFarland Standard (inoculum) f. concentration of antimicrobial being tested
When the activity of a combination of antimicrobials is substantially greater than the activity of the single most active one. The effect is: a. antagonistic b. synergistic c. bacteriostatic d. indifferent
b. synergistic
Extended spectrum beta lactamase (ESBL) testing will be useful as a part of the AST of: a. enterobacteriaceae b. Streptococcus spp. c. Staphylococcus spp. d. Enterococcus spp.
a. Enterobacteriaceae
The ‘D’ test will test for the resistance of which antimicrobial among S. aureus isolates. a. vancomycin b. clindamycin c. oxacillin d. linezolid
b. clindamycin
Ampicillin treatment will acheive adequate levels in: a. blood b. urine c. CSF d. A and B e. A and C f. A, B, and C
f. A, B, and C
The microorganism responsible for Rocky Mountain Spotted Fever is: a. chlamydia trachomatis b. coccidiodes immitis c. histoplasma capsulatum d. rickettsia rickettsii
d. rickettsia rickettsii
True or False: Molds produce fluffy, cottony, wooly or powdery colonies on media and can be identified by the morphology of conidia and their arrangement on their hyphae.
True
This organism is the etiological (causative) agent of the most common STD in the United States: a. chlamydia trachomatis b. cryptococcus neoformans c. treponema pallidum d. candida albicans e. histoplasma capsulatum
a. chlamydia trachomatis
This extracellular, infective form that resembles a gram negative bacillus is part of the life cycle of Chlamydia trachomatis. a. host cell b. elementary body c. blastoconidia d. conidia
b. elementary body
The budding cells of candida albicans, consisting of a mother and daughter cell, may be described as: a. pseudohyphae b. hyphae c. elementary bodies d. blastoconidia
d. blastoconidia
The etiological agent of lyme disease is: a. rickettsia rickettsii b. chlamydia trachomatis c. treponema pallidum d. borrelia burgdorferi e. coccidiodes immitis
d. borrelia burgdorferi
This fungal organism is implicated in a clip of a case study on blackboard last week from monsters inside me. It causes valley fever. a. Rhizopus sp. b. Candida albicans c. Aspergillus fumigatus d. Microsporum canis e. Histoplasma capsulatum f. Coccidiodes immitis
f. Coccidiodes immitis
A request was submitted for the direct examination of skin tissue for the presence of fungi. Which is the best staining method to utilize? a. gram stain b. calcofluor white c. India ink d. darkfield e. lactophenol blue
b. calcofluor white
True or False: Room temperatures greater than 85 degrees F will increase the reactivity of RPR results
True
This organism can be described as: a. zygomycete b. dimorphic c. dermatophyte d. yeast
a. zygomycete
Yeast produce asexually by the production of these structures. a. arthroconidia b. blastoconidia c. pseudohyphae d. chlamydoconidia
b. blastoconidia
Bird (Niger) seed agar is useful in the identification of which of the following? a. C. neoformans b. C. albicans c. C. immitis d. S. schenckii
a. C. neoformans
Treponemes may be detected in material from skin lesions using this staining method. a. gram stain b. calcofluor white c. india ink d. darkfield
d. darkfield
The media that would not be used for the primary recovery of fungi is: a. SABHI agar b. dermatophyte test medium c. cornmeal agar d. brain-heart infusion agar
c. cornmeal agar
True or False: Candida sp., other than albicans, will not have germ tubes but may have pseudohyphae, with constrictions between the parent cell and the pseudohyphal projections
True
Chronic localized fungal infections that result from traumatic implantation are described as: a. subcutaneous b. systemic c. opportunistic d. cutaneous
a. subcutaneous
These colonies were observed on SABHI media at 25 and 35 degrees C. The ID is most likely: a. Histoplasma capsulatum b. Candida albicans c. Aspergillus fumigatus d. Microsporum canis
b. Candida albicans
This organism is implicated in allergic reactions. Rarely causes human infections. a. H. capsulatum b. Penicillium sp. c. Rhizopus sp. d. M. canis
b. Penicillium sp.
True or False: An organism that appears as yeast at 25C and as a mould at 25C can be described as dimorphic.
False
All of the following are necessary for mold identification except: a. Microscopic morphology b. API 20C Aux c. Macroscopic/colony morphology d. growth rate
b. API 20C Aux
The formation of these germ tubes indicates the organism is: a. C. neoformans b. C. tropicalis c. C. immitis d. C. albicans
d. C. albicans
A good selective, primary media to recover Microsporum canis from a scalp sample would be: a. bird seed agar b. Sab dex - SABHI c. Dermatophyte test media d. Cornmeal
c. Dermatophyte test media
A colony from a positive blood culture had the following gram stain. A germ tube was performed and was positive after 5 hours. The germ tube results should be reported as: a. unable to interpret, repeat b. negative - not likely C. albicans c. positive - likely C. albicans
a. unable to interpret, repeat
Infections with Candida albicans are generally considered: a. systemic b. subcutaneous c. superficial d. opportunistic
d. opportunistic
Which of these is not a feasible diagnostic test to diagnose Rocky Mountain Spotted Fever. a. Serology b. PCR c. Immunohistology d. Culture
d. Culture
Which of the following is a dimorphic fungi? a. H. capsulatum b. C. albicans c. C. neoormans d. A. fumigatus e. M. canis
a. H. capsulatum
All of the following are associated with Chlamydia trachomatis infection except: a. meningitis b. pelvic inflammatory disease c. ocular trachoma d. Lymphogranuloma venereum (LGV)
a. Meningitis
True or False: A patient was diagnosed with syphillis based on a positive FTA-ABS test. After successful treatment the FTA-ABS results will return to negative.
False
The method of choice to diagnose Cryptococcal meningitis in US hospitals is: a. calcafluor stain b. india ink stain c. germ tube production d. capsular antigen detection
d. capsular antigen detection
Laboratory workers should always work under a biological safety hood when working with cultures of: a. C. albicans b. C. immitis c. C. neoformans d. Candida species (other than albicans)
b. C. immitis
Based on colony morphology, this organism can be described as: a. Hyaline b. Yeast-like c. Dematiaceous d. Bacterial
c. Dematiaceous
A reactive RPR confirms the presence of this in the serum: a. Treponemal antibody b. Syphillis antigen c. Hemagglutinins d. Reaginic antibody
d. Reaginic antibody
The appropriate specimen to collect for recovery of this organism is: a. Feces b. Skin scrapings c. Blood d. Urine
b. Skin scrapings
The required culture media for Mycoplasma pneumonie is: a. brain heart infusion b. sheep blood agar c. biphasic SP-4 d. SABHI
c. biphasic SP-4
The rounded structures attached to the ends of the pseudohyphae were seen on cornmeal agar of a culture of candida albicans. They are: a. chlamydoconidia b. hyphae c. germ tubes d. conidiophores
a. chlamydoconidia
This organism is implicated in severe infections of nasal mucosa, face, sinus and brain. a. Aspergillus b. Penicillium sp. c. Coccidiodes sp. d. Rhizopus sp.
d. Rhizopus sp.
Chlamydia trachomatis is suspected in a male being seen in the ER. A urine specimen is collected. Which is the most appropriate test to order? a. Chlamydia culture b. Serology (IgM) c. Nucleic Acid Amplification (PCR/AMPA) d. Fluorescent antibody (DFA)
c. Nucleic Acid Amplification (PCR/AMPA)
All of these organism are associated with infection by inhalation, except: a. Coccidiodes immitis b. Aspergillus fumigatus c. Candida albicans d. Histoplasma capsulatum
c. Candida albicans
These microscopic structures are best demonstrated using this media: a. inhibitory mold agar b. bird seed agar c. cornmeal agar d. dermatophyte test media
c. cornmeal agar
This colony has been isolated from a skin culture. Microscopic features can best be seen by performing. a. germ tube test b. india ink preparation c. darkfield exam d. lactophenol cotton blue wet mount
d. lactophenol cotton blue wet mount
True or False: Mycoplasmas differ from other bacteria because they have no cell wall.
True
What is the organism or appropriate reservoir or vector responsible for transmission to humans. Microsporum canis
dogs and cats
What is the organism or appropriate reservoir or vector responsible for transmission to humans. Cryptococcus neoformans
pigeons and other birds
What is the organism or appropriate reservoir or vector responsible for transmission to humans. Borrelia burgdorferi
ticks