Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Biology Lab > Final Practical Exam > Flashcards

Final Practical Exam Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

Gene

A

A section of DNA that codes for a particular trait. One from each parent.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Alleles

A

Alternate genes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Locus

A

A specific location

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Errors during DNA replication and Mutations

A

Result from environmental agents such as UV light, nuclear radiation or certain chemicals.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Restriction Enzymes

A

Show differences in DNA sequences between individuals.

Molecules produced by bacteria that attach to DNA at particular sites.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Electrophoresis

A

DNA fingerprinting involves the analysis of DNA fragments of different sizes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Recognition Sites

A

Where the molecules attach to DNA and cleave it.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Which pole do DNA migrate to?

A

Positive pole; place them on the negative side.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How do different sizes migrate in agarose?

A

Larger molecules do not move well. Smaller molecules have less friction and travel farther down the agarose.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Concentration and pores in agarose

A

The higher the concentration of agarose, the smaller the pores.
The lower the concentration, the larger the pores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the best resolution for electrophoresis

A

lower voltages and longer run times.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

agarose solution concentrations

A

x% = x g/100 ml

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Power supply

A

Emits electrical current

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Buffer

A

Carries electrical current

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Comb

A

Creates wells in gel

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Enzymes

A

Proteins that act as catalysts for biological reactions that bind directly to a substrate molecule.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Substrate

A

Catechol

18
Q

enzyme used

A

potato, polyphenoloxidase extract

19
Q

Why use a spectrophotometer with enzyme catalysis?

A

When oxidized, catechol produces o-quinone and produces colored products. Can be measured by Absorbance.

20
Q

Enzymes and temperature

A

When hot, denatures, no results.
When cold, very slow reactions due to low energy.
When RT, reacts normally.

21
Q

Catabolic process

A

The breaking down of complex substances into simpler ones in living organisms, with the release of energy.

22
Q

Cellular Respiration

A

The series of metabolic processes by which living cells produce energy through the oxidation of organic substances

23
Q

Fermentation

A

The process by which complex organic compounds, such as glucose, are broken down by the action of enzymes into simpler compounds without the use of oxygen.

24
Q

Formula for Cellular Respiration

A

C6 H12 O6 + 6 O2 –> 6 CO2 + 6 H20 + Energy (including ATP)

25
Q

Formula for Alcoholic Fermentation

A

C6 H12 O6 –> 2 C2 H5 OH + 2 CO2 + Energy

26
Q

Monosaccharides

A

metabolic fuels like glucose and fructose

27
Q

Disaccharides

A

sucrose

28
Q

polysaccharides

A

starch

29
Q

What gets oxidized

A

Succinate oxidizes to Fumarate

30
Q

What gets reduced

A

FAD reduces to FADH2

DPIP also gets reduced

31
Q

Color of DPIP

A

When oxidized, blue.

When reduced, colorless.

32
Q

Photosynthesis

A

Synthesis of complex organic materials from CO2, H2O using sunlight as the source of energy and with the aid of chlorophyll and associated pigments.

33
Q

Formula for Photosynthesis

A

6 CO2 + 6 H2O + Light –> C6 H12 O6 + 6 O2

34
Q

Where does photosynthesis take place?

A

In the chloroplasts of eukaryotes

In cell membranes of some prokaryotes

35
Q

Light reactions

A
  • Convert solar energy to chemical energy in form of NADPH and ATP
  • Take place in thylakoid membrane
  • H2O is split
36
Q

Light independent reactions

A
  • Convert CO2 to glucose using chemical energy.

- Take place in stroma of the chloroplast.

37
Q

Pigments in chloroplast

A

Chlorophyll a
Chlorophyll b
Xanthophylls
B-Carotene

38
Q

Polar

A

Exhibiting polarity; an aqueous solvent, or chromatography paper.

39
Q

Non-polar

A

Not polar; Organic solvent

40
Q

Polarity on paper

A

Polar pigments will stick to the paper and non-polar pigments will be dissolved.

Biology Lab (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions