final lecture Flashcards
serum
the liquid portion of blood after clotting has occured
plasma
the liquid portion of blood without the cells when blood is spun down in a tube with anticoagulant
what is the primary function of the anticoagulant sodium fluoride?
antiglycolytic agent
inhibits glycolysis
choice of anticoagulant for hematology
EDTA
choice of anticoagulant for coagulation
sodium citrate
choice of anticoagulant for chemistry
heparin
why is freezing whole blood not normally recommended to preserve a specimen
the red cells will lyse causing hemolysis
what are the two primary reasons patients are placed in isolation rooms?
to protect healthcare staff and visitors from infections
to protect an immunocompromised patient
how does EDTA prevent clotting?
by binding/chelating calcium in the form of potassium or sodium salt
how does heparin prevent clotting?
inhibits conversion of prothrombin to thrombin
what are the capillary puncture sites on an adult?
the index, middle, or ring finger.
where would you not want to perform capillary puncture on adult?
the middle of the finger
what is the preferred area on a finger for puncture?
perpendicular to the fingerprints on the outside of the finger
what is a hematoma and what causes it?
- collection of blood under the skin - indicative of poor technique
- going through a vein
- partially inserting needle into vein
- grazing side of the vein
state the consequences of pushing a tube onto the needle before entering the patient’s vein.
loss of vaccum = no suction in tube
could contaminate tube
explain the purpose of removing the tube from the needle prior to withdrawing from the patient’s vein.
to release the vaccum in the tube
what are clot activators and some examples
substance that inhibits or enhances coagulation
ex. glass/silica/siliceous earth/ thrombin
explain the order of draw
the order of draw is the list of when to draw specific tubes in a specific order
why does the order of draw exist?
this exists to prevent the cross contamination of additives between tubes
what does a sclerosed vein feel like when palpated?
hard/cord like
should a sclerosed vein ever be used for veinipuncture?
no
what is edema?
swelling
what is fistula?
abnormal connection between artery and vein
why are fistulas important to consider when deciding on site selection for venipuncture?
they are used for dialysis patients, so cannot be used
explain what a bevel is & what abnormalities to look for
bevel is the slanted tip on hollow venipuncture needle
ex. bent/curved/shaved/burns/spurs
why are therapeutic drug monitoring tests often timed collection tests?
to measure the peak and trough levels because people metabolize drugs at different rates and to monitor the amount of drugs at certain times to maximize effectiveness and prevent toxicity
what is the name of the heel bone?
calcaneus
what does skin puncture blood most closely resemble?
arterial blood
explain why routine or strict isolation is used.
patient is isolated to prevent the spread of a communicable disease
what is the PPE used in routine and strict isolation?
gown, masks, gloves
explain protective or reverse isolation.
patient is isolated to protect from infection (severely immunocompromised patients)
what PPE is used in protective or reverse isolation?
gown, masks, gloves, hair net, booties
what does an abnormal retraction of a blood vessel wall during venipuncture cause?
vein collapse
what is the most important thing you should do before performing any type of specimen collection on a patient
wash hands and ID patient