Final Exam Questions Flashcards
What is a control?
The purpose of a control is to set a standard and compare it to experimental results
What does an unpaired T-test look for?
an unpaired T test looks for the difference between means of two unrelated groups
What does a paired T-test look for?
a paired T test compares the means of the same group under two separate scenarios
When do you reject the null hypothesis?
reject it when p is less than or equal to 0.05 because there is a significant difference, meaning t is greater than or equal to 0.05
When do you accept the null hypothesis?
accept it when p is greater than 0.5 because there is no significant difference, meaning t is less than 0.5
how do you adjust the light intensity on a microscope?
using the light knob aka the iris diaphragm
What was the non competitive inhibitor in the enzyme experiment?
phenylthiourea was a noncompetitive inhibitor
what is used to test for starch?
the iodine test: brownish turns dark blue or purple
if you completely digest starch, what do you get?
glucose
what 2 things denature proteins?
pH and temperature
what is benedict’s test? what if it is negative?
benedicts test for carbohydrates, reducing sugars: turns red orange (does not test for starch or sucrose); if it is negative it stays blue
what is used to test for lipids?
Sudan’s test; bright red color
Biuret tested for?
Biuret tested for protein, turned purple if positive
what is the monomer of proteins?
amino acids
what is the monomer of lipids?
fatty acids and glycerol
what is the monomer of carbohydrates?
monosaccharides
what is the monomer of nucleic acids?
nucleotides
what are facts about enzymes? what dont they do?
- they are catalysts
- they show specificity for certain molecules
- they can be regulated by effectors and inhibitors
- they are recyclable
- they dont provide activation energy
difference between prokaryotes and eukaryotes
- prokaryotes are unicellular, eukaryotes are multi-celled
- Dna in prokaryotes is in the cytoplasm, DNA in eukaryotes is in the nucleus
- Prokaryotes are Bacteria and Archaea
- Eukaryotes are animals, plants, fungi, protists
differences between animal cells and plant cells
- plant cells, fungi, prokaryotes have cell walls
- animal cells do not have cell walls or chloroplasts
- animal cells have a centrosome and a lysosome
- plant cells have a large central vacuole
- animal cells have a cytoskeleton
- both have a cell membrane
what four things do all cells have?
all cells have chromosomes, cytoplasm, cell membrane, and ribosomes
where does salt go in a semi-permeable membrane between two salt solutions, one 10% and one 80%?
salt will go from high to low to try to balance it out
where does water go in terms of high and low solute concentrations?
water goes from low solute concentration to high solute concentration because if there are high amounts of salt, it wants to lessen it so it goes over there
what is hypertonic
hypertonic has a higher solute concentration of fluid
what is hypotonic
hypotonic has a lower solute concentration of fluid
what has less solute hypertonic or hypotonic?
hypotonic
what happens if a cell is placed in a hypertonic solution?
a hypertonic solution would have more salt, so the water goes out following the salt, and the cell shrivels
what happens if a cell is placed in a hypotonic solution?
if a cell is placed in a hypotonic solution, theres more salt in the cell so it swells up and bursts (cytolysis)
what would happen to a contractile vacuole if you put an amoeba in DI water or salt solution?
in DI water, it would pump faster
in salt solution, it would go slower
What passed through the bag and what didn’t in the dialysis experiment?
salt and glucose passed through the bag but protein and starch were too big
did water or iodine move in the dialysis experiment?
water moved because the bag got heavier, and iodine moved because the bag turned blue
how many cells are at the end of mitosis and are they identical?
2 and they are genetically identical