final exam - PS 7-10 + quiz Flashcards
MHC is a ________
group of genes that code for proteins crucial for the immune system
why is RT-PCR and gel electrophoresis a useful way of detecting the SARS-Cov-2 virus in infected tissue?
because RT-PCR is extremely sensitive, meaning it can detect even the smallest amounts of a virus
its also highly specific reducing chance of false positive
also it uses RNA which covid is an mRNA virus
gel electrophoresis is like the verification part
autoinducer quorum-sensing
bacteria sends out signals to each other based on their density
- the biofilm that forms on teeth
which molecule’s levels oscillate during the cell cycle?
cyclins
infinium microarrays
can simultaneously analyze thousands of SNP markers across the entire genome
- also cost effective & reliable
which molecule is a useful drug target against viruses like HIV?
reverse transcriptase
epistasis F2 ratio
9:3:4
Myostatin D
Transcription factor that grows muscles to the right size
- kick it out and u get super muscle (like blue Belgian cattle)
Mutation in sickle cell anemia
Single amino acid has been changed
Allelic replacement
Introduce the target vector gene and if ever a break, homologous recombination will integrate that gene (also antibiotic resistance gene in the intron? - that recombinase enzyme might delete and then you have a scar)
Doogie mice
Mice engineered to express certain variants of the NMDA receptor - they learned much faster than the controls
allelic replacement example
Cell specific knockouts using cre recombinase
Transgenic mouse expressing Cre recombinase ( recognizes loxP sites around the gene of interest and cut it out
- most cells don’t turn on the Cre enzyme though
- but activate it in some cells and then you have certain cells that are genetically edited
