FINAL EXAM 4

Question 1

TRUE OR FALSE. A base pair consists of a Purine bonded to a purine.

Answer 1

False

Question 2

True or False. Purines are larger than Pyrimidines.

Answer 2

True

Question 3

1. Purine
2. 2 hydrogen bonds
3. Pyrimidine
4. 3 hydrogen bonds

Answer 3

1. 2 cyclic rings
2. A,T
3. 1 cyclic ring
4. G,C

Question 4

____ Makes dna from dna.

Answer 4

DNA Polymerase

Question 5

Where does transcription take place?

Answer 5

in the nucleous

Question 6

what happens in transcription?

Answer 6

process of making RNA

Question 7

what carries out the process of transcription?

Answer 7

RNA polymerase

Question 8

transcription proceeds ____?

Answer 8

translation

Question 9

RNA differs from DNA because RNA is?

Answer 9

Single stranded, not helical, replaces thymine with uracil, dependent on rna polymerase.

Question 10

What is the function of mRNA?

Answer 10

Delivers the DNA message to the ribosomes in the form of RNA.

Question 11

Translation occurs in/at the?

Answer 11

nucleolus, cytosol, rough ER.

Question 12

true or fasle. Translation is the proccess of making protien under the direction of mRNA.

Answer 12

TRUE.

Question 13

As an amino acids are bonded at the ribosomes a polypeptide starts to form, this process is called ?

Answer 13

Elongation

Question 14

True or False. rRna grabs the appropriate amino acid during translation.

Answer 14

False.

Question 15

True or False. all types of RNA are made as a result of translation.

Answer 15

False

Question 16

mRNA has a sequence of the nucleotide that code for an amino acid , this sequence is referred to as?

Answer 16

Codon

Question 17

What is the sequence in the process of making protein?

Answer 17

DNA > tRNA, rRNA> Protien

Question 18

The lac operon is a/an ____ operon.

Answer 18

Inducible

Question 19

the Lac regulatory gene codes for a/an ____ ____?

Answer 19

active, repressor

Question 20

The segment of a bacterial gene that controls transcription is a/an?

Answer 20

operon

Question 21

what end product is produced by the 3 genes regulated by the lac operon?

Answer 21

beta Galactosidase

Question 22

the ___ on the lac operon promotes the binding of RNA polymerase.

Answer 22

Promoter

Question 23

true or false. If lactose is available the lac operon will be turned off.

Answer 23

false

Question 24

which of the following is/are involved with human gene control?

Answer 24

carbs, hormones, operons

Question 25

true or false. protein kinase is responsible for phosphorylation .

Answer 25

True.

Question 26

true or false. non steroid hormones are the first messengers the active G proteins.

Answer 26

true

Question 27

some bacteria have non-essential circular DNA called?

Answer 27

Plasmid

Question 28

which of the following functions to cut DNA?

Answer 28

restriction enzyme, ecori

Question 29

which of the following represents the foreign DNA inserted into a plasmid?

Answer 29

recombinant dna

Question 30

____ reads DNA and makes RNA.

Answer 30

Rna polymerase

Question 31

PCR is used to?

Answer 31

amplify dna

Question 32

____ is used to visualize a DNA fingerprint.

Answer 32

gel electrophoresis

Question 33

one gene therapy technique uses ___ to deliver dna.

Answer 33

viruses

Question 34

bacteria reproduce by means of ?

Answer 34

binary fission

Question 35

true or false. RT-PCR stands for real time polymerase chain reaction.

Answer 35

true

Question 36

True or false. Dna is made from protien.

Answer 36

false.

Question 37

1. restriction enzyme 2. protein 3. circular dna 4. dna ligase

Answer 37

1. ecori 2. dna polymerase 3. plasmid 4. dna glue

Question 38

Who built the first model of the double helix?

Answer 38

1953 James Watson and Francis Crick

Question 39

what is reverse transcriptase?

Answer 39

converts viruses rna to dna.

Question 40

In DNA fingerprinting there are RFLP'S and VNTR. what is the difference between them?

Answer 40

``` Variable number tandem repeat Used for Identification and Inheritance There are 2 Classes of VNTR’s Microsatellites Less than 5 Base Pairs Minisatellites 10 - 60 Base Paris Found in the Centromere and on Telomeres Telomeres = TTAGGG ``` RFLP’s Random Fragment Length Polymorphisms When the tandem repeats are amplified and ran threw a gel a unique pattern or DNA fingerprint is formed

Question 41

what is the cloning vector ?

Answer 41

The vector is the bacteria and the gene is cloned.

Question 42

what are primers?

Answer 42

sections of rna attached to dna

Question 43

what way is dna read?

Answer 43

3 to 5 prime