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Biology 181 > FINAL EXAM 4 > Flashcards

FINAL EXAM 4 Flashcards

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1
Q

TRUE OR FALSE. A base pair consists of a Purine bonded to a purine.

A

False

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2
Q

True or False. Purines are larger than Pyrimidines.

A

True

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3
Q
  1. Purine
  2. 2 hydrogen bonds
  3. Pyrimidine
  4. 3 hydrogen bonds
A
  1. 2 cyclic rings
  2. A,T
  3. 1 cyclic ring
  4. G,C
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4
Q

____ Makes dna from dna.

A

DNA Polymerase

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5
Q

Where does transcription take place?

A

in the nucleous

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6
Q

what happens in transcription?

A

process of making RNA

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7
Q

what carries out the process of transcription?

A

RNA polymerase

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8
Q

transcription proceeds ____?

A

translation

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9
Q

RNA differs from DNA because RNA is?

A

Single stranded, not helical, replaces thymine with uracil, dependent on rna polymerase.

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10
Q

What is the function of mRNA?

A

Delivers the DNA message to the ribosomes in the form of RNA.

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11
Q

Translation occurs in/at the?

A

nucleolus, cytosol, rough ER.

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12
Q

true or fasle. Translation is the proccess of making protien under the direction of mRNA.

A

TRUE.

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13
Q

As an amino acids are bonded at the ribosomes a polypeptide starts to form, this process is called ?

A

Elongation

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14
Q

True or False. rRna grabs the appropriate amino acid during translation.

A

False.

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15
Q

True or False. all types of RNA are made as a result of translation.

A

False

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16
Q

mRNA has a sequence of the nucleotide that code for an amino acid , this sequence is referred to as?

A

Codon

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17
Q

What is the sequence in the process of making protein?

A

DNA > tRNA, rRNA> Protien

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18
Q

The lac operon is a/an ____ operon.

A

Inducible

19
Q

the Lac regulatory gene codes for a/an ____ ____?

A

active, repressor

20
Q

The segment of a bacterial gene that controls transcription is a/an?

A

operon

21
Q

what end product is produced by the 3 genes regulated by the lac operon?

A

beta Galactosidase

22
Q

the ___ on the lac operon promotes the binding of RNA polymerase.

A

Promoter

23
Q

true or false. If lactose is available the lac operon will be turned off.

A

false

24
Q

which of the following is/are involved with human gene control?

A

carbs, hormones, operons

25
Q

true or false. protein kinase is responsible for phosphorylation .

A

True.

26
Q

true or false. non steroid hormones are the first messengers the active G proteins.

A

true

27
Q

some bacteria have non-essential circular DNA called?

A

Plasmid

28
Q

which of the following functions to cut DNA?

A

restriction enzyme, ecori

29
Q

which of the following represents the foreign DNA inserted into a plasmid?

A

recombinant dna

30
Q

____ reads DNA and makes RNA.

A

Rna polymerase

31
Q

PCR is used to?

A

amplify dna

32
Q

____ is used to visualize a DNA fingerprint.

A

gel electrophoresis

33
Q

one gene therapy technique uses ___ to deliver dna.

A

viruses

34
Q

bacteria reproduce by means of ?

A

binary fission

35
Q

true or false. RT-PCR stands for real time polymerase chain reaction.

A

true

36
Q

True or false. Dna is made from protien.

A

false.

37
Q
  1. restriction enzyme
  2. protein
  3. circular dna
  4. dna ligase
A
  1. ecori
  2. dna polymerase
  3. plasmid
  4. dna glue
38
Q

Who built the first model of the double helix?

A

1953 James Watson and Francis Crick

39
Q

what is reverse transcriptase?

A

converts viruses rna to dna.

40
Q

In DNA fingerprinting there are RFLP’S and VNTR. what is the difference between them?

A 
Variable number tandem repeat
Used for Identification and Inheritance
There are 2 Classes of VNTR’s
Microsatellites
Less than 5 Base Pairs
Minisatellites
10 - 60 Base Paris
Found in the Centromere and on Telomeres
Telomeres = TTAGGG

RFLP’s Random Fragment Length Polymorphisms
When the tandem repeats are amplified and ran threw a gel a unique pattern or DNA fingerprint is formed

41
Q

what is the cloning vector ?

A

The vector is the bacteria and the gene is cloned.

42
Q

what are primers?

A

sections of rna attached to dna

43
Q

what way is dna read?

A

3 to 5 prime

Biology 181 (2 decks)

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