FInal 4 Flashcards
Provide mechanical strength in cells
IF
Used for cell locomition and cell surface shape
Actin
Determine position of organelles and direct movement
MTs
Specialized structures that increase absorptive surface area in epithelial cells
Microvilli
What kind of cytoskeletal material are nuclear lamina made from?
IFs
Cytoskeletal material used for flagella
MTs
Birthplace of all cytoplasmic MTs
MTOC
What are MTs protofilaments?
Polymers of alpha-beta tubulin heterodimers
Which subunit of MT does not cleave GTP to GDP?
Alpha
How many MT protofilaments assemble to form a tube?
13
What do actin monomers bind?
ATP
How many actin protofilaments intertwine to form a fiber?
2
What do two monomers of IFs form?
Coiled-coil dimer
What do two coiled-coil dimers of IFs form?
Staggered tetramer in an antiparallel arrangement
How many protofilaments of IFs twist into a ropelike filament?
8
What is the rate-limiting step for actin filament assembly?
Nucleation
What is the nucleation process of filament assembly dependent upon?
Concentration
Free actin and tubulin monomers are bound by what?
NTP
What happens when a GTP cap is lost?
MT will fray and shrink
Name for when MTs shrink due to loss of GTP cap
Catastrophe
MTs regrowing after a catastrophe
Rescue
What does taxol do to MTs?
Stabilizes them, essentailly killing rapidly dividing cells
Complex responsible for initiating MTs
gamma-TuRC
What end of MTs are inside MTOC
Negative
Complex responsible for initiating actin arrays
Arp2/3
Where do Arp2/3 usually nucleate?
PM
What angle do Arp2/3 bind to sides of existing actin filaments to form branches?
70
2 actin monomer binding proteins
Thymosin, profilin
Actin filament destabilizing protein
cofilin
Actin filament severing protein
Gelsolin
2 actin filament capping proteins
CapZ, tropomodulin
MT binding protein
Stathmin
MT filament binding protein
MAPs
2 MT filament destabilizer
Catasrophin, katanin
Actin-based motor
Mysoin
MT-based plus-end directed motor
Kinsein
MT-based negative-end directed motor
Dynein
WHat happens when myosin is treated with trypsin?
Heads and a small piece of tail are released from the tail
What happens when myosin is treated with papain?
Splits the heads up and removes the tail
Which myosin moves towards the minus end?
Mysoin Vi