final Flashcards
(lab safety) are you allowed to wear contact lenses while in the lab?
no; only allowed with approved goggles
(lab safety) each person must wear ___ of some kind while in the lab.
glasses
(lab safety) ____ shoes must be worn, ___ are not allowed. shorts, tanks, frilly/flared sleeve are ___ allowed. what is strongly recommended to wear?
low heeled;
open toed;
not;
lab apron/lab coat
(lab safety) everyone should be familiar with the location of the ____, ____, ____, and ____.
fire extinguisher, eye wash, shower, fire blanket
(lab safety) what do you do if a chemical gets in your eye?
use eye wash immediately and seek doctor
(lab safety) chemical burns should be flushed out immediately with ____. if a large area was exposed use the ______. who do you inform of the incident?
water;
safety shower;
the nearest person to contact the lab instructor (doctor if necessary)
(lab safety) if come into contact with fire, you must flush the burn area under ____ and immediately see _____.
cold water;
doctor
(lab safety) is long hair accepted in the lab?
no
(lab safety) what should you do before the experiment? what concepts should you know? (4)
read and study the experiment;
hazards involved, proper precautions to observe, how to handle/dispose of hazardous materials, proper use of equipment
(lab safety) all chemicals are presumed poisonous, if in contact with skin _____ before they are transferred to the mouth.
wash throughouly
(lab safety) can you put your mouth on a pipet? what can you use?
no;
aspirator bulb/ long clean hose
(lab safety) what should you do when heating a test tube?
point it away from yourself and others, oscillate tube to prevent bumping
(lab safety) may you leave a reaction unattended?
no
(mass spect) what is mass spectrometry?
a technique in which a sample is ionized through a beam of energetic electrons
(mass spect) the electrons cause _____ and break chemical bonds producing _____ which are _____ and ____.
ionization, positive ions, sorted and detected
(mass spect) what is their separation based on?
based on their mass to charge ratio
(mass spect) ions with a more positive charge will be ____ towards the detector
repelled faster
(mass spect) how do scientist utilize mass spectrometry in the industry? in research? in medicine?
they use it to analyze the purity of their compounds after synthesis;
they use it to analyze the identity of their compounds after an experiment;
utilized to characterize and sequence different proteins or (in genomics) they characterize single nucleotides/short tandem repeats
(mass spect) how do you calculate M+? what does it signify?
M+ = n(mass)+n2(mass)
n = moles of atom
mass = atomic weight
M+ gives the most accurate molecular weight for a given molecule
(mass spect) when calculating M+ what’re some key point in calculating?(3)
- always use the entire number of the correct isotope (never average)
- always write out the entire number for each component/answer
- always write answer in units of amu
(mass spect) calculate M+ of C6H6?
78.046950192 amu
(mass spect) calculate M+ of C6H12?
84.09390038 amu
(mass spect) calculate the M+ of C3H8O?
60.05751488 amu
(mass spect) how do you calculate M+1? what does it signify?
M+1 = [(isotope % natural abundance)/(normal % natural abundance)] x n x 100% + ()
n = moles of atom
() = repeat calculation separately for each atom and add
the percentage of M+ peaks that come form one atom changing in one isotopic number
(mass spect) when calculating M+1 what’re some important concepts to consider? (3)
- always use/write the entire number of the correct isotope, no averaging
- always write answer in form of a percent
- know changing isotope # doesn’t apply to Br or Cl
(mass spect) calculate M+1 of C6H6?
6.57945047%
