final Flashcards
order of GI
Title, Abstract, Intro, Methods, Results, Discussion, References
what to have in title
latin name
statement regarding experiment and it’s outcome
what to have in abstract
“spoiler of paper”
essentially summarizes whole paper by addressing the main reason for the investigation
-hypothesis
-study system
-results and interpretation
-concluding remarks on outcome
NO REFERENCES
Introduction
general to specific
-introduces the topic and science behind it
-other studies done in topic area (references)
-followed by ur study/ hypothesis and importance
methods
-describe whole lab
-mention 3 replications
-describe how data is to be summarized and presented
results
-graph
-biological trend in data
-refer to figure!
-figure heading at bottom
do not explain findings ! ex. as expected
discussion
specific to general
-conclusion regarding hypothesis
-support ideas with literature
don’t list data!
-address anamalies
-ways you could improve
references
in alphabetical order (hanging indent)
organic helper molecule
coenzymes
inorganic helper molecules
cofactors
enzymes
speed up reaction by lowering activation energy
substrate
molecule acted on by the enzyme
active site
where enzyme binds
rutabaga extraction to find what
peroxidase ( function to break down hydrogen peroxide)
what is used in a dye coupled reaction
guaiacol
colorless then becomes orange/brown when oxygen reacts with it
outcome of enzyme experiment
-with each concentration as time went on absorbance increased
-higher concentrations had higher absorbance
cell resp equation and where it takes place
C6H12O6 + 6O2 -> 6CO2 + 6H2O + ATP (32-38)
mitochondria
photosynthesis equation and where it takes place
energy (photon) + 12H2O + pigments + 6CO2 -> carbohydrate + 6O2 + 6H2O
chloroplasts
how to separate pigments
chromatography
solvent front
furthest distance travelled by solvent
DCPIP
when accepts electrons (reduced) - blue to colorless
DCPIP spinach extract results
absorbance decreases in tubes with DCPIP over time as the DCPIP goes from blue to colorless as it accepts electrons from photosynthesis
how to get Rf
distance travelled by substance / distance travelled by solvent front
best to metabolize yeast
glucose
produces most CO2