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Bacteriology 2 > Fermenters > Flashcards

Fermenters Flashcards

1
Q

Media that is used to detect fermentation/acid production with Bromthymol blue as indicator

A

OF Media of Hugh and Leifson

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2
Q

Media that is used to detect fermentation/acid production with Phenol red as indicator

A

OF Media - CDC method

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3
Q

How many tubes are used in OF Media of Hugh and Leifson?

A

Two (2) tubes

Open (aerobic) and closed (anaerobic)

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4
Q

OF Media of Hugh and Leifson:

  1. Positive result:
  2. Negative result:

a. Yellow
b. Blue green/Green

A
  1. Positive result – a. Yellow
  2. Negative result – b. Blue green/Green
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5
Q

Interpretation of result: OF Media of Hugh and Leifson

Open/Closed

  1. +/+
  2. +/-
  3. -/-

a. Oxidizer/Non-fermenters
b. Non-oxidizers/Non-saccharolytic
c. Fermenter

A
  1. +/+ = Fermenter
  2. +/- = Oxidizer/Non-fermenters
  3. -/- = Non-oxidizers/Non-saccharolytic
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6
Q

How is Triple Sugar Iron dispensed?

A

As slant & butt

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7
Q

Oxygen requirement of Enterobacteriaceae

A

Facultative anaerobe

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8
Q

All Enterobacteriaceae are non-fermenters.

a. True
b. False

A

b. False.

All are fermenters.

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9
Q

Usual media to detect Enterobacteriaceae’s ability to produce acid (ferment)

A

Triple Sugar Iron

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9
Q

What are the three (3) fermentable carbohydrates in TSI and their corresponding measurement?

A
  1. Glucose (1 part)
  2. Lactose (10 parts)
  3. Sucrose (10 parts)
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10
Q

TSI media can also be used as H2S indicators.

a. True
b. False

A

a. True

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11
Q

How can TSI show H2S production?

A

TSI has two (2) H2S indicators: Ferrous sulfate and sodium thiosulfate

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12
Q

Match: TSI

  1. Acid pH
  2. Alkaline pH

a. K
b. A

Slant/Butt color:

  • Yellow
  • Red
A
  1. Acid pH - A - Yellow
  2. Alkaline pH - K - Red
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13
Q

Manner of reporting of TSI result

A

Slant/Butt

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14
Q

Match the results:

  1. K/K
  2. K/A
  3. A/A

a. Non-lactose fermenter
b. Lactose fermenter
c. Non fermenter

Slant/Butt Color:

  • Yellow
  • Red
A
  1. K/K - Non fermenter - Red/Red
  2. K/A - Non-lactose fermenter - Red/Yellow
  3. A/A - Lactose fermenter - Yellow/Yellow
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15
Q

E.coli, Enterobacter, Klebsiella are what type of fermenters?

A

Lactose fermenters

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16
Q

Shigella spp., Proteus, Providencia and Morganella are what type of fermenters?

A

Non-lactose fermenters

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17
Q

Citrobacter, Y. enterocolitica, and S. sonnei are what type of fermenters?

A

Late lactose fermenters

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18
Q

P. aeruginosa is what kind of fermenter?

A

Non-fermenter

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19
Q

Two possible results of Enterobacteriaceae in TSI

A
  1. K/A
  2. A/A
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20
Q

Four (4) tests that will differentiate Salmonella-Shigella

A
  1. Motility test
  2. H2S production
  3. Malonate
  4. Decarboxylase test
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21
Q

Salmonella or Shigella:

  • Motile, H2S (+), Malonate and Decarboxylase (+)
A

Salmonella

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22
Q

Salmonella or Shigella:

Nonmotile, H2S (-), Malonate and Decarboxylase (-)

A

Shigella

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23
Q

Salmonella and Shigella are what kind of pathogens?

a. Respiratory
b. Oral
c. Genital
d. Intestinal

A

d. Intestinal pathogens

Both are agents of diarrheal disease

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24
Q

Salmonella and shigella are never normal flora.

a. True
b. False

A

a. True

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25
Q

These characteristics will separates Vibrio spp. from the Genus Aeromonas.

A

Vibrio spp. - String test (+); Susceptible to Vibriostatic Agent

Aeromonas - String test (-); Resistant to Vibriostatic Agent

26
Q

Vibriostatic agent is also known as

A

0/129 agent

27
Q

Selective differential media for Vibrios

A

Thiosulfate Citrate Bile salt Media (TCBS)

28
Q

pH Indicator of TCBS

A

Thymol blue
Bromthymol blue

29
Q

What is the carbohydrate present in TCBS?

A

Sucrose

30
Q

Color of colonies that are sucrose fermenters

A

Yellow

31
Q

Color of colonies that are non-sucrose fermenters

A

Green

32
Q

Enrichment media for Vibrios

A

Alkaline Peptone Water

33
Q

Vibrio spp. that are non-sucrose fermenters

A

V. mimicus,
V. parahaemolyticus,
V. vulnificus

(MPV)

34
Q

Vibrio spp. that are sucrose fermenters

A

V. alginolyticus
V. cholerae,
V. fluvialis
V. furnissi
V. metschnikovii

(ACFFM)

35
Q

All Vibrios are halophilic, except:

A
  1. V. cholerae
  2. V. mimicus

Both are non-halophilic

36
Q

How much salt concentration is required for Vibrios?

A

8-10%

37
Q

Other term for Beta-hemolysis due to TDH

A

Kanagawa phenomenon

38
Q

Media used to demonstrate Kanagawa phenomenon of V. parahaemolyticus

A

Wagatsuma agar

39
Q

Component of Wagatsuma agar

A

High salt mannitol concentration

40
Q

Causative agent of Summer Diarrhea in Japan

A

V. parahaemolyticus

41
Q

Causative agent of Rice watery stool

A

V. cholerae

42
Q

Cholera is what kind of disease, and characterized by?

A

Diarrheal disease characterized by Rice watery stool

43
Q

What is TDH?

A

Thermostable Direct Hemolysis

44
Q

Characteristic of Vibrio that will separate them from Enterobacteriaceae

A

Vibrio - Oxidase (+)
Enterobacteriaceae - Oxidase (-)

45
Q

Characteristic of Vibrio that will separate them from Plesiomonas

A

Vibrio - Inositol (-)
Plesiomonas - Inositol (+)

46
Q

Characteristic of Vibrio that will differentiate them from Pseudomonas

A

Vibrio - Fermenters (all glucose fermenters; lactose (-) except V. vulnificus
Pseudomonas - Non-fermenters

47
Q

Vibrio spp. that is less virulent, occupational hazard → continuous contact with sea water (fishermen, sailor)

A

V. alginolyticus

48
Q

Most significant vibrio

A

V. cholerae

49
Q

Species that is Triple Decarboxylase negative (-)

A

Pantoea agglomerans

50
Q

Pantoea agglomerans is negative to which amino acids?

A

Negative to Lysine, Ornithine and Arginine

51
Q

Species positive in Triple Decarboxylase

A

Pleisomonas spp.

52
Q

This test is done to detect if the organism produces decarboxylase enzyme

A

Decarboxylase test

53
Q

What are the two (2) ways to do Decarboxylase test?

A
  1. Moeller’s broth
  2. LIA
54
Q

Carbohydrate and indicator present in Moeller’s broth

A

Glucose
Bromcresol purple

55
Q

Color result in Decarboxylase test

A

(+) purple
(-) yellow

56
Q

What does decarboxylase enzyme do in decarboxylase test?

A

Decarboxylase enzyme removes carboxyl group from the amino acid

57
Q

Match: Decarboxylase Test

Amino acids
1. Lysine
2. Ornithine
3. Arginine

Carboxyl Group
a. Putrescine
b. Citrulline
c. Cadaverine

A
  1. Lysine - Cadaverine
  2. Ornithine - Putrescine
  3. Arginine - Citrulline
58
Q

Campylobacter spp. that appears S-shaped or wings of seagulls

A

C. jejuni and C. coli

59
Q

Motility of C. jejuni and C. coli

A

Darting motility

60
Q

Growth requirement of Campylobacter jejuni and Campylobacter coli

A
  1. Capnophilic
  2. Microaerophilic
61
Q

The virulence factor of which organism is production of urease?

A

Helicobacter pylori

62
Q

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A

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Bacteriology 2 (9 decks)

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