Fatty acid metabolism and biosynthesis

Question 1

Fatty acyl CoA (ox)

Answer 1

Oxidation
Acyl CoA dehydogenase
Trans enoyl CoA
FAD - FADH2

Question 2

Trans enoyl CoA (ox)

Answer 2

Hydration
Enoyl CoA hydratase
Hydoxyacyl CoA
- H20

Question 3

Hydroxyacyl CoA (ox)

Answer 3

Oxidation
Hydroxyacyl CoA dehydrogenase
Ketoacyl CoA
NAD - NADH

Question 4

Ketoacyl CoA (ox)

Answer 4

Thiolysis
Ketoacyl CoA thiolase
Fatty acyl CoA (2C shorter)
+ CoAsH

Question 5

Step 1 biosynthesis

Answer 5

Acetyl-CoA - malonyl CoA

Acetyl-CoA carboxylase

+HCO3- , ATP-ADP+Pi

Question 6

Step 2 biosynthesis

Answer 6

Linkage of acetyl CoA and malonyl CoA to ACP by transacylase

Question 7

Step 3 biosynthesis

Answer 7

Condensation

Acetyl-ACP + Malonyl-ACP to Acetylacetyl-ACP

B-ketoacyl-ACP synthase (KS)

• CO2+ACP

Question 8

Step 4 biosynthesis

Answer 8

Reduction

Acetoacetyl ACP to D-B-hydroxybutyryl-ACP

B-ketoacyl-ACP reductase (KR)

-NADPH

Question 9

Step 5 biosynthesis

Answer 9

Dehydration

D-B-hydroxybutyryl-ACP to a,b-transbutenoyl-ACP

B-hydroxyacyl-ACP dehydrogenase (DH)

-H20

Question 10

Step 6 biosynthesis

Answer 10

Reduction

a,b-transbutenoyl-ACP to butyryl -ACP

enoyl-ACP reductase (ER)

-NADPH

Question 11

What is the final product of FA biosynthesis, how many runs through?

Answer 11

Final product palmitoyl-ACP

7x runs

Question 12

How is palmitoyl-ACP converted to palmitate fatty acid?

Answer 12

Hydrated via enzyme palmitoyl thioesterase.