Factors Affecting Enzyme Activity

Question 1

What are the 2 ways of measuring rate of reaction?

Answer 1

How fast the product is made

How fast the substrate is broken down

Question 2

What are the factors that affect enzyme activity?

Answer 2

Temperature

pH

Substrate concentration

Enzyme concentration

Question 3

If temperature gets too high, why does the reaction stop?

Answer 3

Enzyme is denatured and active site changes shape

Question 4

If pH gets too high, why does the reaction stop?

Answer 4

Enzyme is denatured and active site changes shape

Question 5

Why is the reaction faster when there is a higher substrate concentration?

Answer 5

More substrate molecules means a collision between substrate and enzyme is more likely

Question 6

What happens if substrate concentration becomes too high?

Answer 6

All active sites become full

Question 7

Why is there a faster reaction if you increase enzyme concentration?

Answer 7

More likely enzyme molecules will collide with substrate molecules

Question 8

What are the 2 types of enzyme inhibitors?

Answer 8

Competitive

Non-competitive

Question 9

What are competitive inhibitors?

Answer 9

Molecules that have a similar shape to that of a substrate so bind to active site but no reaction takes place

(Block active site so substrate can’t fit in)

Question 10

What are non-competitive inhibitors?

Answer 10

Molecules that bind to enzyme away from its active site which causes the active site to change shape