Factors Flashcards
1
Q
pH
A
- alter interaction bt R groups;
- ionic, H bond disrupted;
- denature enzyme 3D confirmation;
- lost active site;
=>non functional catalytic and contact residues
2
Q
@ Low temp
A
Low KE;
Low rate of collision
3
Q
@ increasing KE
A
- increase effective collision bt enzyme and substrates;
- more ES complex form;
- more energy; rxtant collide; more likely to overcome Ea barrier to form pdt
4
Q
@opt temp
A
Max;
Rate of effective collision is highest;
Largest amt of pdt formed per unit times
5
Q
@ temp higher than opt
A
Thermal agitation disrupts bonds stabilising 3D;
Altering active site;
No longer fit to substrate;
Denature and lose catalytic fn
6
Q
Low enzyme conc
A
More active site available;
Successful collision bt increases;
More ES complex;
Higher rate
7
Q
At higher enzyme conc
A
No enough substrate to occupy active sites;
No increase in ES complex formation;
Substrate conc becomes LF
