Enzyme Specificity

Question 1

Explain lock and key

Answer 1

Substrate conformation is complementary to conformation of active site;

Collision and correct orientation;

ES complex;

Pdt release

Question 2

General description of induced fit

Answer 2

Initial conformation not complementary to the shape of the substrate molecules

Question 3

Explain induced fit

Answer 3

Substrates combine with enzyme at active site;

Induced conformational change;

Active site becomes catalytically active

Question 4

Why active site becomes catalytically active?

Answer 4

aa forming active site moulded into precise conformation and position;

Stretches critical binds, substrate fit more snugly into active site;

Stabilise transition state struct’;