Extra Flashcards
Inversion mutation
Segment of a chromosome is reversed end to end
Coding strand vs template strand
DNA
Template: 3’-5’ (copied during mRNA synthesis- start at 3’ end, but 5’ on mRNA)
Coding: 5’-3’ (same base sequence as mRNA but T replaced w/ U)
Translocation
Rearrangement of parts between non-homologous chromosomes
Silent mutations
No significant change in phenotype
Taq polymerase
Greatly amplifying short sequences of DNA in PCR
Lactose Operon
Operon (segment of genes controlled by same promotor) required for the transport and metabolism of lactose in E. coli
Lac Operon- lactose present
Lac repressor cannot bind to operator
RNA Polymerase can attach
Repressor
A DNA or RNA binding protein that inhibits the expression of genes by binding to the operator
binding repressor blocks the attachment of RNA polymerase to the promoter preventing transcription
Lac Operon no lactose
Repressor binds to operator and RNA polymerase cannot attach
Inducer
A molecule that regulate gene expression can bind to repressors or disabled repressors
the gene is expressed because RNA polymerase can attach