Experimental Techniques

Question 1

What is the purpose of ELISA testing?

Answer 1

Quantify antigens or antibodies in liquid samples

Question 2

what is the visualisation used for ELISA testing?

Answer 2

• Spectrophotometric measurement (not visual)

Question 3

how are the results imaged of an ELISA test?

Answer 3

• Graphs or chart showing optical densities corresponding to antigen/antibody concentrations

Question 4

In situ hybridisation purpose:

Answer 4

to detect specific DNA/RNA sequences in tissue sections

Question 5

visualization of ISH

Answer 5

chromogenic or flourescent signals under a microscope

Question 6

result imaging of ISH

Answer 6

Colorimetric or flourescent signals showing distribution of nucleic acids within cells or tissues

Question 7

purpose of WEstern blotting:

Answer 7

Detects specific proteins quantities in homogenized tissue samples

Question 8

visualization of Western Blotting

Answer 8

Electrophoresis followewd by transfer to a membrane and detection using antibodies

Question 9

Immunohistochemistry purpose

Answer 9

Detects specific proteins in tissue sections

Question 10

result imaging of IHC

Answer 10

colorimetric or flourescent staining indicating protein localization with the tissue structure