Experimental approach Flashcards
1
Q
Pharmacological inhibition
A
f
2
Q
Pharmacological activation
A
f
3
Q
siRNA inhibition
A
siRNA are not completely specific, and .
Advantages:
KO before protein synthesis.
Limitations:
Off-target effects.
Knock down dependent on transduction efficacy.
Toxic to cells, especially primaries.
4
Q
Stimulation
A
f
5
Q
Forced exercise
A
f
6
Q
Reduce partial pressure
A
f
7
Q
Photolytic uncaging
A
f
8
Q
Timed conditional KO
A
f
