experiment 4 Staph vs Sal (FOOD POSION

Question 1

What is one of the most common forms of food poisoinging caused by

Answer 1

entero-toxin producing strains of Staphylococcus aureus

Question 2

does staphylococcous aureus release a exotoxin that is heat stable

Answer 2

yes

Question 3

what are the two commonways that food illnesses occur

Answer 3

1) Staphylococcus aureuos
-releases a heat stable exotoxin into food
-it is an entero-toxin producing thing
-large amount needed to be toxic (cause food poisoingin)
2)Salmonella
-injesting this pathogenic microorganism because it can grow in the intestinal tract and produce toxic substances
-releases endotoxins from the the LPS in oute membrane of the organism

Question 4

Is only one organism usually the etiologic agent

Answer 4

yes
An etiologic agent is a factor, such as a microorganism, chemical substance, or environmental condition, that is responsible for causing a disease or pathological condition.

Question 5

Method

Answer 5

week 1

Innoculate sample a and b of hamburger dilutions (one is staph one is salmonella) on to rappaport (allows for salmonella detection) and make two plates. (one for a and B( of dilution on staph plates

week 2
look for growth on the plates, growth means it is staph

Question 6

What is rappaport vassiliadis and why is it used

Answer 6

rappaport allows for the growth of salmonella but not staphylococcus

Question 7

What is usually done with rappaport vasiadialis but asnt done in our lab

Answer 7

usually tetrathionate brilliant green enrichment browhts and brillienat green sulfa and macconkey agars are used

-this allows for enrichment for the growth of salmonella

Question 8

Esssneutally how was staphylococcus tested for
and how was salmonella

Answer 8

1)using staphylcoccus 110 plate
2)Coagulase Test
-culture in brain heart infusion broth is added to reconstituded plasma
-it gets thick due to enzyme coagulase which is also produced by those that produce the exotoxin

salmonella
1)rappaport vasiadialis
2)MacConkey Plate
3)triple sugar iron agar

Question 9

TSI (triple sugar iron agar) typical salmonella results

Answer 9

1) Lactose/ sucrose utilization:
-pos yellow
neg: red (SALMONELLA)
2)Dxtrose utilizatio
-pos: yekkiw (w or w/o gas) (SAL)
if black, then it is positive
-negative: no change
3)H2S
pos: black (salm)
neg: no black
4) gas:
pos(sal)
neg

Question 10

what was the results obtained

Answer 10

B was salmonella
A was Staphyloccocus