ex 12: denitrificiation Flashcards
1
Q
nitrate reduction into
A
nitrite, ammonia, nitrous oxide (N2o) or N2 if its complete
2
Q
if anaerobic
A
denitrification
if aerobic
ammonification may occur
3
Q
method
A
typotone yest extract nitrate broth
pesudomonias aeruginosa
look for gas production, means complete denitiriication has occured
if no gas test for nitrite (tromsdorff and sulfuric acid) as well as nitrate (diphenylamine)
if no results, then its prob ammonia
4
Q
why is tryptone yeast nitrate broth used
A
tryptone: porvides essential amino acids
yeast: needed for growth of stuff
nitrate: the electron acceptor, what isneeded for dentieificay
