EXPERIMENT 4 Factors Affecting enzyme Activity Flashcards
increase the reaction rate without changing the free energy.
Catalysts
kinds of enzymes
o Oxidoreductase (NAD, NADH+)
o Ligase
o Lyase
o Transferase (Vitamin B6)
o Hydrolase
o Isomerase
the reactant upon which the enzyme acts.
substrate
An enzyme contains an ____ that binds the substrate, forming an enzyme-substrate complex.
active site
The ___ releases the product/s once the reaction has occurred.
catalyst
Measure of rate at which the enzyme converts the substrate to products in a biochemical reaction.
ENZYME ACTIVITY
Factors that Affect Enzyme Activity:
o Temperature
o pH
o Substrate Concentration
o Enzyme Concentration
reacts with starch to form a starch or iodine complex, which gives a
characteristic blue color to the reaction mixture.
Iodine
o Test Tube 1: _____
o Test Tubes 2 to 4: ______
o Test Tube 5: _____
positive control
samples to check the activity
negative control
Add drops of saliva samples to test tubes _____
two (2) to four (4).
o Test Tube 1: _____
o Test Tubes 2 and 3: _____
o Test Tube 4: _____
o Test Tube 5: _____
Blue-Black (starch not digested)
Dark Blue
Light Brown
Gold (starch digested)
has an optimum pH of 7, so the activity of the enzyme at pH 7 is
at the maximum; thus, it will exhibit a _____ solution since the starch is
fully digestive.
alpha- amylase; yellow color
an inhibitor of -amylase, which will exhibit a blue-colored solution since no starch is digested.
Silver nitrate
Temperature is Lower than the Optimum
decreased catalytic activity due to insufficient energy for collisions.
temperature is Higher than the Optimum
enzyme denaturation may happen, thus inactivating the enzyme.
