EXPERIMENT 2 Colorimetric assay of proteins (quantitative)

Question 1

After reading the absorbance, throw all the solutions in a beaker and properly discard the solution in the _____-

Answer 1

organic waste bottle.

Question 2

The absorbance must be read within an ___ of the
addition of the Bradford reagent

Answer 2

hour; kc it may degrade

Question 3

Measures the concentration of total protein in a sample

Answer 3

Bradford protein assay

Question 4

what is the principle of bradford protein assay

Answer 4

binding of protein molecules to coomassie dye under acidic conditions resulkt in brown to blue

Question 5

Bradfird reagent conatins what

Answer 5

Coomassie Brilliant Blue G-250

Question 6

CBBG dye contains

Answer 6

2 sulfonic acid and 6 phenyl groups interacting w proteins w positive charge(basic, histidine, arginine and glycine, lysine) residues and hydrophobic residues (9)

Question 7

Advantages of CBBG

Answer 7

use of reducing agent is compatible w bradford assay
quixk inexpensive and simple
it can be used in high molecular weight proteins

Question 8

Disadvantages of CBBG

Answer 8

cant be used in detergents
applicable to certain protein sequences only

Question 9

most commonly used standard due to its sensitivity

Answer 9

Bovine Serum Albumin

Question 10

Wavelength range of UV-Vis

Answer 10

190- 900nm

Question 11

parts of cuvette

Answer 11

frosted side- holding
transparent side- measuring

Question 12

small rectangular tube to hold aqueous samples

Answer 12

cuvette, can be plastic glass or quartz

Question 13

refernfce or compartment contain just the solvent

Answer 13

blank run or auto zeroing

Question 14

axis for concdntration and absorbance

Answer 14

x and y