EXPERIMENT 4-7 IH

Question 1

common immunohematologic reaction

Answer 1

-HEMAGGLUTINATION
-HEMOLYSIS

Question 2

most common immunohematologic reaction

Answer 2

HEMAGGLUTINATION

Question 3

sample used in experiment 4

Answer 3

5% red cell suspension

Question 4

disruption or destruction of rbc membrane

Answer 4

hemolysis

Question 5

what is added to 5% rcs to yield positive reacTION FOR HEMOLYSIS

Answer 5

distilled water

Question 6

what is added to 5% rcs to yield negative rESULT FOR HEMOLYSIS

Answer 6

NSS

Question 7

this is termed as the clumping together of the rbcs resulting from interaction of rbc antigen to antibody

Answer 7

HEMAGGLUTINATION

Question 8

interpret: intact cell button with clear supernatant

Answer 8

NO HEMOLYSIS

Question 9

absence of cell button with red supernatant

Answer 9

complete hemolysis

Question 10

presence of cell button with pink supernatant

Answer 10

partial hemolysis

Question 11

interpret w+

Answer 11

tiny agglutinates turbid background

Question 12

why is microscopic examination necessary

Answer 12

• to differentiate pseudoagglutination from true agglutination
• to determine mixed field reaction
• to confirm a negative reaction

Question 13

false positive sources of error

Answer 13

-clotted specimen
-bacterial contamination
-metal contamination of NSS
-dirty glasswares
- overcentrifugation
-overreading
-contaminated AHG

Question 14

false negative sources of errors

Answer 14

• inadequate washing of red cells
  -inadequate incubation
• old serum sample
• serum/AHG not added
• underfilling
• undercentrifugation
• cell suspension too weak/too heavy reactivity
  -non-reactive AHG

Question 15

developed gel method

Answer 15

DR. YVES LAPIERRE

Question 16

advantage of uing gel method

Answer 16

-standardization
-smaller amount
-no need for washing

Question 17

discovred ABO blood group

Answer 17

KARL LANDSTEINER

Question 18

Most important of all blood group

Answer 18

ABO system

Question 19

most immunogenic in transfusion practice

Answer 19

ABO SYSTEM

Question 20

true or false:
ABO BLOOD GRP is the only blood group in which individuals have antibodies in their serum to antigens that are absent in their rbcs

Answer 20

TRUE

Question 21

A.K.A cell typing

Answer 21

forward grouping

Question 22

A.K.A serum typing

Answer 22

reverse grouping

Question 23

what is being detected in forward grouping

Answer 23

antigen

Question 24

what is being detected in reverse grouping

Answer 24

antibody

Question 25

sample in forward grouping

Answer 25

red cell suspension

Question 26

sample in reverse grouping

Answer 26

serum

Question 27

reagent in forward grouping

Answer 27

antisera

Question 28

reagent in reverse grouping

Answer 28

known A1 CELLS AND B CELLS

Question 29

advantage of reverse grouping

Answer 29

highly recommended method for blood typing (specificity)

Question 30

naturally occuring ntigens

Answer 30

ABH IgM

Question 31

immune antibody

Answer 31

IgG

Question 32

ABH antigens develop on what day of fetal life

Answer 32

37th day

Question 33

what month can ABO be detected in serum

Answer 33

3-6 months

Question 34

peak of ABO antibodies detected in serum

Answer 34

5-10 years

Question 35

when will ABO antibodies declines

Answer 35

65 years of life

Question 36

how many percent of individuals possess Se genes

Answer 36

78%

Question 37

test for ——— may establish the true ABO group of an individual whose rbc antigens are poorly developed

Answer 37

ABH SECRETION

Question 38

SUCH SECRETED ANTIGEN CAN BE DEMONSTRATED IN SALIVA BY what test

Answer 38

inhibition test with ABH AND LEWIS antisera