Experiment 3: Basic Principles of Antigen antibody testing

Question 1

foreign molecules that bind specifically to an antibody or TCR

Answer 1

antigens

Question 2

located on the red cells and protrude from the cell membrane

Answer 2

RBC antigen

Question 3

protein in nature qith high molecular weight that is b9th antigenic and immunogenic

Answer 3

carrier

Question 4

nonprotein with low MW and is antigenic but not immunigenic

Answer 4

hapten

Question 5

carrier plus hapten

Answer 5

complete antigen

Question 6

concentration and half life of IgM

Answer 6

10 % 5 days

Question 7

cause intravascular hemolysis bc of efficiency in complement

Answer 7

IgM

Question 8

components of pretransfusion testing

Answer 8

1. ABO typing
2. Rh Typing
3. antibody screen
4. antibody identification
5. Crossmatching

Question 9

acute HTR happens within

Answer 9

<24 hrs

Question 10

causes extravascular destruction through pahgocytosis

Answer 10

IgG

Question 11

clear immune complexes, engance phagocytosis, promote neutrophil degranulation

Answer 11

opsonization

Question 12

increase smooth muscle contraction and inflammation

Answer 12

anaphylaxis

Question 13

kill foreign antigen by membrane rupture

Answer 13

lysis

Question 14

recruit platelets and phagocytes

Answer 14

chemotactic

Question 15

indicative of antigen antibody recognition

Answer 15

agglutination and hemolysis

Question 16

two stages of agglutination

Answer 16

sensitization
lattic formation

Question 17

factors that affect sensitization

Answer 17

temperature
incubation time
pH
ionic strength

Question 18

reduces ionic strength of RBC

Answer 18

LISS

Question 19

factors that affect lattice formation