EXPERIMENT 1 POST LAB Flashcards
Controls the angle and mount of sunlight sent to the objective
Aperture diagram
Used to eliminate yellow color emitted by the tungsten
Blue filter
It the least expensive objective. Partially corrects for chromatic and spherical aberrations.
Achromatic objective
One with two oculars
Binocular microscope
It uses transmitted light and lenses. Objects appear dark against a white background. It is used for most routine clinical work
Brightfield microscope
One with 2 lens system – objective and ocular
Compound microscope
It focuses light on specimen
Condenser
Distance throughout which all parts of the specimen are in focus simultaneously
Depth of focus
Limits the area of illumination to image the field
Field diaphragm
Area of specimen that can be seen
Field of view
Used to help objective gather light from a wide numerical aperture. Provides high resolution.
Immersion oil
What type of viscosity is used in Immersion oil?
Type B
Method of focusing and centering light path and spreading light uniformly. Ensures optimum contrast and resolution.
Kohler illumination
Magnification x magnification objective. 1000 x highest magnification achievable with bright field microscope.
Magnification, total
Mathematical expression of light admitted by lens. The higher the NA, the greater the resolution
Numerical aperture
Lenses attached to revolving nosepiece. Most commonly used are low power (10x), high poer (40x) and oil immersion (50 or 100x)
Objectives
Eye piece, usually 10x
Ocular
Object in center or field at 1 magnification will be in center of field at other magnification
Parcentric
Object remains in focus from 1 magnification to another
Parfocal
More expensive objective that corrects curvature of field. Results in flat field with uniform focus
Planachromatic objective
Ability to reveal fine detail and distinguish between 2 close points
Resolution
Light control knob. Light intensity shouldn’t be regulated by condenser of diaphragms
Rheostat
Type of bulb used for brighfield microscopy
Tungsten-halogen bulb
Image seen through microscope. Upside down and reversed.
Virtual image
Distance between slide and objective. Decreases with higher magnification objectives.
Working distance.
