Exp 8 PCR

Question 1

PCR stands for

Answer 1

polymerase chain reaction

Question 2

A primer is…

Answer 2

A short piece of DNA that is complementary to the known DNA sequence

Question 3

Which enzyme binds to the primer to extend its strand by incorporating free nucleotides?

Answer 3

DNA polymerase

Question 4

DNA is negative or positive?

Answer 4

negative

Question 5

What region would typically be sequenced?

Answer 5

Variable

Question 6

why we microwave MO

Answer 6

weaken the cell wall
release the DNA
break open the cells
Denaturation

Question 7

If your DNA fragment size is known to be relatively small, the concentration of your agarose should be adjusted to be

Answer 7

Relatively higher

Question 8

A piece of agarose gel should be prepared with

Answer 8

Agarose powder, EtBr, and TAE buffer

Question 9

what are the tree steps of PCR

Answer 9

denaturation (95°C), annealing (55°C), extension (72°C)

Question 10

how to find the number of replications

Answer 10

2^le nombre de cycle