exp 4 Iron Flashcards
what do we use slope to find?
E, molar absorptivity bc slope is E x b
what is beers law?
A= E b c a is absorbance e is molar absorptivity, (L/mol x cm) b is path length or distance photons travel c is concentration (mol/L)
when are more photons absorbed?
as concentration increases
if photons traverse a longer path length
if the absorption probability is high
what does beers law show?
absorbance is linearly proportional to concentration of the absorbing entity
variables in c1v1=c2v2
c1 is unknown or stock
v1 is from the pipet
c2 is from the equation of the line
v2 is from the volumetric flask
how do we prep the iron?
need to reduce iron III to iron II
4 Fe3+ + 2 NHOH = 4 Fe 2+ + N2O + 4 H+ + H2O
prep of calibration curve?
set spectrometer to max absorption wavelength
solving for x?
x in y= mx + b is the concentration
linear equation
eb= m