Exercise #23 (Diagnostic Immunology)

Question 1

Define:
antibody

Answer 1

immune proteins

Question 2

Define:
antigen

Answer 2

chemicals that initiate an immune response

Question 3

What is a lateral flow chromatographic assay used for?

Answer 3

tests that use antibodies to detect chemical of interest
ex hCG hormone in pregnancy test

Question 4

In the rapid strep test used in class:
o Describe the 3 types of antibodies used in the test.
 What does the antibody bind to?
 Is the antibody labeled?
 Does the antibody move during the test?

Answer 4

First bind to Streptococcus pyrogens when strip is dipped into mixture, second unlabeled version of anti-Streptococcus antibody Third is an anti-rabbit antibody made of goats the control line to endure that the antibodies made it the way up
antibody does not move in a positive test it will “catch” the moving complexes of streptococcus antigens that are bound to the labeled anti- Streptococcus,

Question 5

What does a positive result look like?

Answer 5

Will have two lines

Question 6

What does a negative result look like?

Answer 6

Will have one line at the top

Question 7

What does the control line tell you? What does it mean if it doesn’t appear?

Question 8

THE END