exercise 2 Flashcards
Define diffusion and identify physiological examples of diffusion
Passive random movement of molecules with their concentration gradient. Respiratory gasses (oxygen and carbon dioxide) and ions move by diffusion. Glucose moves into cells by facilitated diffusion (requires insulin)
How does the diffusion of a gas like oxygen differ from the diffusion of an ion like sodium
Oxygen is lipid soluble so it passes through the phospholipid barrier without the help of a carrier or ion channel
Define osmosis and identify physiological examples of osmosis
The passive movement of Water molecules through a porous membrane with their concentration gradient (from hypotonic region) all movement of water Into and out of individual cells is by osmosis. Movement out of kidney tubules back into blood stream is by osmosis
Define filtration and identify physiological examples of filtration
Movement of molecules through a porous membrane using hydrostatic forces. Molecules leave capillaries by filtration
Why is it possible to demonstrate diffusion osmosis and filtration in experiments that do not include living tissue
doesnt need cellular energy in the form of atp
How does the experiment demonstrate diffusion
Potassium permanganate passively moved with concentration gradient
The result of this experiment suggests the two agar plates have different permeability. What would make membranes in your body have different permeability
The type and distribution of ion channels present
How does this experiment demonstrate the influence of time on the rate of osmosis
Connect change in volume gain and rate of volume change decreased as time passed. Because the gradient gradually decreased
Could this experiment also demonstrate the influence of the size of the concentration gradient on the rate of osmosis explain
Yes as water moved into the dialysis bag the contents became more diluted which decreased the gradient
Because the salt molecules cannot pass through the dialysis tubing, the concentration of the solutions on each side of the dialysis to being can never be the same but a state of equilibrium can still be achieved explain
Because water molecules are in random motion some water molecules are also leaving the dialysis bag. Some point the same volume of water will move into the dialysis bag as exits and the net change in volume will be zero
Summarize the results of this experiment
When blood cells are placed in a relatively hypotonic solution they take on water from the surroundings and swell. Enlarged or perhaps ruptured cells block less light increasing transmittance plateau on upper left. Conversely when blood cells are placed in a relatively hypertonic solution they lose volume to the surroundings and shrink. the shrunken cells block more light decreasing transmittance plateau on lower right. cells placed in a relatively isotonic solution exchange water with their surroundings but don’t swell or shrink vertical section of tracing
Import C was a solution in the beaker’s hypertonic hypotonic or isotonic to the solution in the dialysis bags
Hypotonic. Water always moves with its concentration gradient from the relatively hypotonic region towards a relatively hypertonic region
How does this experiment demonstrate filtration
A solution was passed through a selective membrane by hydrostatic pressure. The water and small sugar particles passed through the membrane while the larger charcoal pieces and starch molecules were trapped by the membrane
list two reasons why it is necessary to test both the original solution and the filtrate for sugar and starch
To confirm that you know how to perform the tests and that all reagents are working properly
To have a positive control test for comparison of your unknown results
Was charcoal present in the filtrate explain how you came to this conclusion
No the charcoal can’t be seen with the naked eye. Crackle was present in the filtrate it was all trapped by the filter paper