Exercise 1 Laboratory tools and equipment Flashcards
Approaches and techniques used by classical embryologists (3)
Genetics
Molecular
Imaging
Laboratory tools and equipment utilized by a Developmental Biologist (4):
The Workbench
Dissecting Tools
The Microscope
Microtome
List of the ones found in the room excluded in the manual (Part 1: 8)
____ Uses steam pressure to cook food quickly and sterilize equipment.
____ Keeps items frozen at low temperatures for long-term storage.
____ Removes moisture from substances by heating them in an enclosed space.
____ Ventilates harmful fumes, gases, or dust away from the user during experiments.
____ Provides high temperatures for materials processing, such as heating or melting.
____ Maintains a low-humidity environment to dry and store moisture-sensitive samples or materials.
____ Separates DNA, RNA, or proteins based on size and charge using an electric field through a gel matrix.
____ Removes solvent from a solution by evaporating it, leaving behind the solute.
Pressure Cooker
Chest Freezer
Oven Dryer
Fume Hood
Furnace
Dessicator
Gel Electrophoresis
Evaporator
List of the ones found in the room excluded in the manual (Part 2: 9)
____ Cuts thin slices of specimens for microscopic examination.
____ Maintains a constant temperature for incubation, heating, or thawing of samples.
____ Pure water free from impurities used in various laboratory applications.
____ Measures mass with high precision for accurate weighing of substances.
____ Measures the intensity of light absorption by a substance at different wavelengths for quantitative analysis.
____ Removes moisture from samples by freezing and then evaporating the ice under vacuum, preserving the sample’s structure.
____ Spins samples at high speeds to separate components based on density or size.
____ Heats or processes samples using microwave radiation for various laboratory applications.
____ Provides high-resolution imaging and magnification for detailed examination of microscopic specimens.
Microtome
Water Bath
Distilled Water
Analytical Balance
Spectrophotometer
Freeze Dryer
Centrifuge
Microwave
Advanced Upright Microscope
The workbench:
It should be wiped down with ____ alcohol
The stage of your ____ should also be wiped down with said alcohol
As well as its knobs, instrument tray and any other items you will be using
70% alcohol
Dissecting scope
Dissecting tools:
Examples of dissecting tools (5)
Scissors
Forceps
Knives
Probes
Scalpels
Dissecting tools:
Methods to sterilize dissecting tools (4)
70% alcohol soak
boiling water
UV fluorescent bulb
Autoclave
Dissecting tools:
Alternative method used by other researchers include them sandwiching their instruments between the folds of a _____ that has been soaked in 70% alcohol
paper towel
The microscope:
State the parts of the microscope (13)
Ocular
Ocular focus knob
Nosepiece
Objective
Arm
Stage
Mechanical stage control
Coarse focus
Fine focus
Iris diaphragm control
Condenser
Condenser Focus
Field diaphragm control
The Microtome:
It’s a specialized precision cutting instrument, which accurately and repeatedly slices sections from a block of embedded tissue.
They are typically used to section (3)
Paraffin
Plastic embedded tissues
frozen tissues
The Microtome:
The main parts of a mictorome include (2)
a sharp knife
tissue block
The Microtome:
What are the section thickness for each type of tissue?
wax?
plastic?
frozen?
wax - 4-10 mm
plastic - 0.5 - 3 mm
frozen - 8 - 15 mm
What is the full name of the microtome found in the DBS stockroom?
Manual Rotary Microtome
