Exchange & Transport 3.3. Flashcards
Digestion and absorption (AO1)
During digestion, large biological molecules are ____________________ to smaller molecules that can be absorbed across cell membranes.
hydrolysed
Digestion and absorption (AO1)
Amylase hydrolyses which bonds?
Glycosidic bonds
(in starch)
Digestion and absorption (AO1)
Where is amylase produced in the body?
Salivary glands
Pancreas
Digestion and absorption (AO1)
Starch is hydrolysed into which disaccharide
Maltose
Digestion and absorption (AO1)
Maltase location
Membrane-bound
Digestion and absorption (AO1)
Membrane-bound
This means the enzyme is located in the cell surface membrane of epithelial cells lining the ileum of the small intestine
Digestion and absorption (AO1)
Disaccharidase examples
Maltase
Sucrase
Lactase
Digestion and absorption (AO1)
Disaccharidase location
Membrane-bound
Digestion and absorption (AO1)
Describe the complete digestion of starch by a mammal (4 marks).
1. Hydrolysis;
2. (Of) glycosidic bonds;
3. (Starch) to maltose by amylase;
4. (Maltose) to glucose by disaccharidase/maltase;
5. Membrane-bound (disaccharidase/maltase);
Digestion and absorption (AO1)
Process by which glucose and amino acids are absorbed into the blood via the epithelial cells of the small intestine
Co-transport
Digestion and absorption (AO1)
Role of sodium-potassium
pump in co-transport
Actively transports sodium ions out of epithelial cell into the blood
This lowers the concentration of sodium ions inside the cell
Digestion and absorption (AO1)
TRUE or FALSE
Sodium ions are needed to absorb glucose and amino acids via co-transport
TRUE
Digestion and absorption (AO1)
Name of transport protein that allows sodium ions AND glucose OR amino acids into the epithelial cell
Co-transporter
Digestion and absorption (AO1)
TRUE or FALSE:
Sodium ions and glucose bind to the same site on the co-transporter
FALSE
(they bind to different sites, each with a specific and complementary shape)
Digestion and absorption (AO1)
Epithelial cells lining the ileum of mammals absorb glucose by co-transport with sodium ions. Explain how (3 marks).
1. Sodium ions actively transported from ileum cell into blood;
2. Lowers concentration of sodium ions, so they enter epithelial cells from lumen of small intestine;
(Sodium and glucose bind to co-transporter at different binding sites)
3. Glucose enters cell by facilitated diffusion along with sodium ions
4 This is co-transport;
5. Glucose then leaves epithelial cell and moves into blood via faciliated diffusion;
Digestion and absorption (AO1)
1 ATP hydrolysis releases energy;
2. This energy allows ions to be moved against a concentration gradient
OR allows active transport of sodium ions;
Digestion and absorption (AO1)
Epithelial cells contain ______________ to increase surface area for absorption
microvilli
Digestion and absorption (AO1)
Describe and explain two features you would expect to find in a cell specialised for absorption (2 marks).
1. Folded membrane/microvilli so large surface area for absorption;
2. Large number of co-transport/carrier/channel proteins so fast rate of absorption;
3. Large number of mitochondria so make more ATP by aerobic respiration;
4. Membrane-bound enzymes so maintains concentration gradient (for fast absorption);
Digestion and absorption (AO1)
Section of small intestine where most absorption takes place
ileum
Digestion and absorption (AO1)
Protease examples
Endopeptidase
Exopeptidase
Dipeptidase
Digestion and absorption (AO1)
Bond hydrolysed by proteases
peptide
Digestion and absorption (AO1)
Endopeptidases hydrolyse ____________ peptide bonds within the polypeptide chain
internal
Digestion and absorption (AO1)
Exopeptidases hydrolyse the peptide bonds at the ________________ ends of the polypeptide chain
terminal
Digestion and absorption (AO1)
Products of dipeptidase action
x2 amino acids
Digestion and absorption (AO1)
TRUE OR FALSE
Dipeptidases are produced
in the stomach
FALSE
(They are membrane-bound enzymes located in the cell surface membrane of the ileum)
Digestion and absorption (AO1)
The action of endopeptidases and exopeptidases can increase the rate of protein digestion. Describe how (2 marks).
1. Exopeptidases hydrolyse peptide bonds at the terminal ends of a polypeptide AND endopeptidases hydrolyse internal peptide bonds within a polypeptide;
2. This produces more surface area for faster hydrolysis;
Digestion and absorption (AO1)
Endopeptidases and exopeptidases are involved in the hydrolysis of proteins. Name the other type of enzyme required for the complete hydrolysis of proteins to amino acids.
Dipeptidase
Digestion and absorption (AO1)
Similarities between co-transport of glucose and amino acids
Both require sodium ions to be actively transported out of the epithelia cell into blood;
Both require sodium ions to bind to a co-transporter to help faciliate diffusion;
Both glucose and amino acids enter the blood via facilitated diffusion;
Digestion and absorption (AO1)
Differences between co-transport of glucose and amino acids
Co-transporter for glucose has a different tertiary structure to co-transporter for amino acid
Channel/carrier protein for glucose has a different tertiary structure to channel/carrier protein for amino acid
Digestion and absorption (AO1)
Describe the mechanism for the absorption of amino acids in the ileum (4 marks).
1. Sodium ions actively transported from ileum cell into blood;
2. Lowers concentration of sodium ions, so they enter epithelial cells from lumen of small intestine;
(Sodium and amino acids bind to co-transporter at different binding sites)
3. Amino acids enter cell by facilitated diffusion along with sodium ions
4. This is co-transport;
5. Amino acids then leaves epithelial cell and moves into blood via facilitated diffusion;
Note: these are the same steps as those involved in glucose absorption
Digestion and absorption (AO2)
1. No/less ATP produced OR No active transport;
2. Sodium (ions) not moved (into/out of cell);
3. No diffusion/concentration gradient for sodium (to move into cell with amino acid via co-transport);
Digestion and absorption (AO1)
Bile contains bile salts, which ____________ fat droplets
emulsify
Digestion and absorption (AO1)
Describe two functions of bile salts
1. Emulsify lipids;
2. Increases surface area for
faster lipase activity;
3. Form micelles
Digestion and absorption (AO1)
Emulsification increases the ____________ of lipids for faster hydrolysis by lipases
surface area
Digestion and absorption (AO1)
Lipases hydrolyse triglycerides into ____________
Glycerol &
3 fatty acids
Digestion and absorption (AO1)
Describe the hydrolysis reactions involved in the digestion of triglycerides (2 marks).
1. Breaking of ester bonds;
2. By addition of water;
Digestion and absorption (AO1)
Explain the advantages of lipid droplets following emulsification.
1. Droplets increase surface areas for lipase action;
2. So faster hydrolysis triglycerides
Digestion and absorption (AO1)
Micelles are made up of……….
Bile salts & fatty acids
Digestion and absorption (AO1)
Explain the advantage of micelle formation
Micelles bring fatty acids to lining of the ileum epithelial cell;
This helps maintain concentration gradient of fatty acids;
Makes fatty acids soluble in water;
Digestion and absorption (AO1)
Describe the role of micelles in the absorption of fats into the cells lining the ileum (3 marks).
1. Micelles include bile salts and fatty acids;
2. Make the fatty acids more soluble in water;
3. Bring fatty acids to the lining of the ileum epithelial cells;
4. Maintain high(er) concentration of fatty acids at the lining of the ileum epithelia cells;
5. Fatty acids absorbed by diffusion;