Exam2 Unit 4/5

Question 1

Genome

Answer 1

Total genetic information carried by a cell/organism

Question 2

Proteome

Answer 2

Entire complement of proteins produced by a

cell/subcellular compartment/tissue/organism

Question 3

Proteomics

Answer 3

Systematic study of the proteome – Amounts, modifications, interactions, localization,
functions of all or subsets of proteins

Question 4

Enzymes Features

Answer 4

Catalysts of biological systems
– Chemical transformations
– Energy conversions
– Transport processes

Question 5

How are reactants called

Answer 5

Substrates

Question 6

Are all enzymes proteins?

Answer 6

Mostly proteins

Some catalytically active RNAs —Ribozomes

Question 7

Are enzymes intracellular ?

Answer 7

Both intra and extra cellular

Question 8

What is stereoselective synthesis?

Answer 8

Produce pure enantiomers

Question 9

Perfect condition for enzymes to operate

Answer 9

Room temperature,
Neutral pH –most
Normal pressure
Aqueous solution —most

Question 10

What is(are) the main function(s) of the enzymes ?

Answer 10

Increase reaction speed, 
       i.e. rate of reaction
Reduce activation energy
            Stabilization of transition state (high-  energy species)
 • Do NOT affect extent of a reaction
            Equilibrium  keq unaffected 
• Do NOT affect free energy change 
                     ∆G°’ or ∆G 
• Unchanged after reaction
 • Function under physiological conditions

Question 11

Enzyme Specificity

Single reaction
Group of reactions

Answer 11

Single reaction- urease

Group of reactions- protease