Exam Two Flashcards
What does O stand for in the antigenic formula?
O antigen
What does O stand for in the antigenic formula?
O antigen
What does H stand for in the antigenic formula?
Flagella
What does F stand for in the antigenic formula?
Fimbriae
What does K stand for in the antigenic formula?
Capsule
Neonatal ETEC (in pigs) characteristics
F5, F6, F41
STa, STb
non-hemolytic
Neonatal till 4 weeks ETEC (in pigs) characteristics
F4
LT, STa, STb, EAST1
Hemolytic
Weaning diarrhea ETEC (in pigs) characteristics
F4, F18
LT, STa, STb, EAST1
Hemolytic
Oedema pigs is caused by
VTEC
Virulence factors of VTEC
Adhesion: F18
Exotoxin: VT2e or Stx2e
Endotoxin
How you you breed a pig that can’t get VTEC?
selection of receptor F18 negative animals
When do bovines get ETEC
Animals less than 3 days
What does H stand for in the antigenic formula?
Flagella
What does F stand for in the antigenic formula?
Fimbriae
What does K stand for in the antigenic formula?
Capsule
Neonatal ETEC (in pigs) characteristics
F5, F6, F41
STa, STb
non-hemolytic
Neonatal till 4 weeks ETEC (in pigs) characteristics
F4
LT, STa, STb, EAST1
Hemolytic
Weaning diarrhea ETEC (in pigs) characteristics
F4, F18
LT, STa, STb, EAST1
Hemolytic
Oedema pigs is caused by
VTEC
Virulence factors of VTEC
Adhesion: F18
Exotoxin: VT2e or Stx2e
Endotoxin
When do symptoms appear in pigs with VTEC?
less than 3 wks after weaning
How you you breed a pig that can’t get VTEC?
selection of receptor F18 negative animals
When do bovines get ETEC
less than 3 days of age
When do bovines get EPEC and EHEC
Animals older than one week
When do bovines get Septicemic E. coli
neonates, lack of colostrum
ETEC virulence factors
F5, F41, F17, CS31A
Sta Stb
ETEC in bovines Pathogenesis
Oral uptake
multiplication
Enterotoxin production
secondary septicaemiae
ETEC therapy in bovines on milking farms
Hygiene
Colostrum
Bovine EPEC gene
eae
Bovine EHEC genes
eae + VT (VT1 and or VT2)
E coli septicaemiae pathogenesis in bovines
Uptake per os or umbilical cord
Septicaemiae
Can be hyperacute, acute or slower w/ organ localization
Dog Ecoli Cystitis/Pyometra strains
F12, F13
O: 2, 4, 6, 83
Ecoli in birds serotypes
O: 1, 2, 78
Ecoli in birds clinical appearances Neonatal
Neonatal colibacillosis
Respiratory colibacillosis and septicimae
Peritonitis in layers
What Salmonella affects calves and what are the symtoms?
S. Dublin
Diarrhea, general symptoms
What is the Typhoid Salmonella in pigs?
S. Choleraesuis
non-host specific
What Salmonella affects horses
S. abortus equi
Pathogenesis of S. abortus equi
Per Os- septicemia or invasive
Venereal- Mare aborts of has a poly arthritic foal, Stallion has reduced fertility
S. Dublin in adults causes
diarrhea, general symptoms
abortion/subclinical mastitis
Salmonella in pigeons
Paratyphus
S. Typhimurium var Copenhagen
Acute paratyphus in pigeons happens
mainly during breeding
Chronic paratyphus in pigeons signs
one wing hanging down
intermittent excretion
Typhoid Salmonella in poultry is caused by
S. Pullorum
Fowl typhoid is caused by
S. Gallinarum
Turkey Typoid Salmonella
S. enterica spp. arizonae
What is the zoonotic Salmonella you can get from eggs?
S. Enteriditis
Klebsiella infections are associated with
Equine metritis
Umbilical infections in horses
Dogs (rare) pyometra, cystitis
Mastitis in bovines
What is the causative agent of the the plague?
Yersinia pestis
Yersinia pestis epidemiology
fleas of wild rodents
Endemic areas for Yersinia pestis
Western N america, southern asia, S and W Africa, N-C S. America
What is the major pathogen in Passeriformes
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis
outdoor birdhouses
transmission from wild animals
Lesions associated w/ acute Passeriformes
large spleen with foci of necrosis
enlarged liver
Lesions associated w/ subacute and chronic Passeriformes
very large spleen w/ foci of necrosis
very large liver with foci of necrosis
foci of necrosis on caeca and lungs
What causes enteric red mouth dz in Salmon?
Yersinia ruckeri
Shigella flexneri
seen more in non human primates
Shigella causes
dysentery due to shiga toxin
Proteus mirabilis causes
otitis and cystitis in dog
What other organisms cause Otitis in dogs
Staph pseudintermedius
Malassexia pahcydermatis
What organisms infect ears after tx?
Proteus
Pseudomonas aeruginosa
What is ocassionaly found in guinea pigs that has a natural resistance to ampi/amoxi?
Morganella morganii
Pseudomonas aeruginosa
common in nosocomial infections
facultative pathogenic and symbiotic
P aeruginosa in Cats and Dogs causes
skin infections- pyoderma cystitis otitis externa different purulent processes corneal ulcers
P. aeruginosa in hamsters, guinea pigs, chinchilla and mink causes
pneumonia and septicaemiae
high mortality in farmed mink
origin drinking water
P. aeruginosa in rabbits causes
skin infection, pneumonia
P. aeruginosa in horses causes
keratitis conjunctivitis- secondary infection following topical tx
metritis-vaginitis
mastitis
P. aeruginosa in reptiles causes
necrotic stomatitis
pneumonia
septicaemia
What causes Galliformes in turkeys
p. aeruginosa
Burkholderis is
g-
aerobic
rod
cat +
Glanders is caused by
Burkholderis mallei
Glanders is an ____ pathogen and has to be ______
Equine
Declared
Glanders pathology and pathogenesis
Pathology: suppurative lesions
Pathogenesis: enter pharynx or skin ->spread via lympathic or blood -> nodular lesions
Acute glanders symptoms
fever, nasal discharge, lympahdenitis, freq. fatal
Chronic glanders symptoms
fever and resp probs
skin abcesses
zoonotic
Melioidosis is a reportable dz. It’s causes by
Burkholderia pseudomallei
t/f Melioidosis has a high host range
true
What is equine psuedoglanders caused by?
Burkholderia pseudomallei
How to differentiate between Burkholeria pseudomallei and Burkholderia mallei
Grow on Pseudomonas cepacia agar
-pseudo grows faster and is motile
The genus Francisella
G- small, pleomorphic coccoid/rod non motile obligate aerobic facilitative intracellular very fastidious growth
Francisella tularensis
reportable dz
contagious
wide range of animals
Resivour for Francisella tuarensis
Lagomorphs/rabbits
Virulence factors for Francisella tularensis
capsule
LPS/endotoxin
Pathogenesis of Francisella tularensis
systemic w/ multi organ failure fac intracellular (mainly MO)
Taylorella equigenitalis
g- non motile coccobacillus fac anaerobic obligate symbiont/pathogenic
Taylorella equigenitalis symptoms
Males- none
Females- Contagious Equine Metritis
Taylorella equigenitalis virulence factors
capsule
cell wall lps
How to dx Taylorella equigenitalis?
Amies charcoal transport medium in less than 24 hours, culture on hematin agar
What’s the principle cause of human brucellosis
B. melintensis
How is Brucella mainly spread?
By female animals
- abortion
- fetus and placenta
Pathogenesis of Brucella
Comes through wounds/oral/mucosa
Spread intracellular in MO to lymph
1-6 wk incubation
Target organs
Virulence of Brucella
no capsule no flagella cell wall -smooth colonies -rough colonies thicker PG Erythritol
Carriers for Brucella
When all intracellular bacteria aren’t killed and immunity goes down bacteria are released from cells
-cause abortion or transfer to fetus
Vaccination for Brucella
Need for both humoral and cellular immunity
-AroC mutants and DnaK mutants
Dx of Brucella
SAW or SAT
Rose Bengal
CFT, ELISA
Brucellosis Skin Test
Cytokines
IFN-Y
Both not widely used, false positives
Dx of Brucellosis
ELISA if positive do another ELISA then IFN skin test
Brucella suis
Small non motile G- coccobacilli
obligate symbiotic/pathogenic
resistant in environment but can’t handle sunlight
Is Brucella suis reportable and eradicated in domestic swine?
Yes and yes
What is the resivour for Brucella suis
wild boar
Which biovars for Brucella suis are most pathogenic for pigs?
1,2, 3
Symptoms of Brucella suis
repro arthritis tendovaginitis Bursitis Osteomyelitis Zoonotic, more pathogenic that B. abortus
Pathogenesis of B. Suis
Introduction via skin/mucosa
Multipies in leukocytes
bacterimia after 1-7 wks in MO/NO for 1wk-34mo
localization w/ abscess formation
Brucella melitensis and Brucella ovis
Sheep and goat
Similar to B abortus in bovines
Mainly in mediterranean (Malta fever)
melintensis- zoonotic
Brucella canis
Need rich media for growth Primary isolation up to 4 wks No O antigens Obligate parasitic/pathogen zoonotic
Symptoms of Brucella canis?
long bacteremia, no fever
STD- lots of shit with the vag and testes
Dx and Tx of Brucella canis?
Dx- bacterology and serology
Bordetella
G- Small cocco-bacillus obligate symbiont facultative pathogenic
Where can B. Bronchoseptica be found in animals>
URT- cilliated respiratory epithelium
What does B. bronchiseptica do in dogs
Dog- kennel
Pigs- atrophic rhinitis
Virulence factors for B. bronchiseptica
Adhesins
Biofilms
Toxins- Hemolysin (adenylate cyclase toxin)
Bvg regulun
Bordetella brochiseptica causes what dzs in pigs
non progressive atrophic rhinitis
pneumonic bordetellosis
progressive atrophic rhinitis
Tx and Prev of Atrophic Rhinitis
difficult, no optimal therapy
hygiene, antibacterial, vaccinate sows
dx- cultivation of nose sampling on blood agar
What’s the causative agent of kennel cough/canine infectious treachobronchitis
Bordetella bronchiseptica
Symptoms of B. Bronchiseptica in cats
nasal discharge
tears
sneezing
non productive cough
B Bronchiseptica in horses
seldom, similar to dogs and cats
tx- prevent dust, rest, prevent spores in hay
What causes Coryza in turkeys?
Bordetella avium
At what age do turkeys get Coryza?
1-6wks, can be chronic
Symptoms of Bordetella in turkeys?
high morbidity, low mortality
decreases growth
nasal discharge, sneezing
Tx of Bordetella in poultry
hygiene, keep ages seperate, inactivated vx for mother
Vx for B. bronchiseptica
inactivated vx , 2 inj given 3 wks apart then yearly
life vx: nasal given at 3wks then yearly, fast protection
Tx. for B. bronchiseptica
self limiting, tetracyclines given when needed
aerosolization of antibiotics
Bordetella in Rabbits, Symptoms
nearly all of them are carriers causes nasal discharge, sneezing, ect Can localize in the eye and cause blindness ear infections abscesses
What two pathogens cause bronchopneumonia in Rabbits?
bordetella + P. multocida
How is Klebsiella transmitted in Equine metritis?
stallion, fomites, vaginal specula and biopsy equipment
Moraxella bovis
obligate symbiotic/pathogenic
g- pleomorphic, in pairs or short chains
cat and oxidase +
non-motile
What is causative agent of bovine pink eye?
Moraxella bovis
Virulence factors for Moraxella bovis
Fimbrae to attach to eye
capsule
lps
exotoxins
Pathogenesis of Moraxella bovis
attaches to conjunctiva/coneal epithelium
produces toxin
inflammation
further growth and production of toxin leads to increased susceptibility for other pathogens and dust
Predisposing factors for Moraxella bovis
Flies, Dust
Young Cattle
How is Moraxella bovis transmitted?
Direct contact
flies
Dx Moraxella bovis
Immunoflurescence
Culture- blood agar, hemolytic
PCR
Moraxella bovis is associated with
keraconjuctivitis in bovines
Moraxella ovis is associated with
keraconjunctivitis and pneumonia in bovines
Pasturella multicoda general
g -
obligate symbiotic
capsule types A-E
little resistant in enviroment
Pasturella multocida virulence factors
adhesins
capsule
lps
iron acquisition
Toxins in Pasteurella multocida
RTX
Rho activating toxin
What do high virulent strains for Pasteurella multocida cause and in what species?
olbigate pathogenic: speticamiae
bovines, birds and rabbits
What do low virulent strains of Pasteurella multocida do and in what species
Facultative pathogenic in nose and tonsils cause rhinitis, pneumonia
pigs, bovines, rabbits
What causes snuffles in rabbits?
Pasturella multocida
High pathogenic serovars of P. mulocida in bovines causes
hemorrhagic septicaemiae
Pasturella multocida in pigs causes
atrophic rhinitis and lung pasteurellosis (pneumonia)
What causes fowl cholera?
Pasturella multocida
different suceptabilites for different species of birds
Pathogenesis of Pasturella multocida in poultry
in nose and beak of healthy animals, enters through there or skin lesions causes septicaemia and excretion though nose/mouth- drinking water/feed
Pasturella multocida in cats and dogs is important because of….
its commonly transmitted via bite wounds
Mannheimia hemolytica
obligate symbiotic
capsule types A-F
low enviromental resistance
Mannheimia hemolytica Virulence factors
Dose dependent Leukotoxin: rtx family Transferrin binding proteins endotoxins capsule fimbriae
Mannheimia hemolytica in ruminants
fac. pathogenic
pneumonia
sometimes septicemiea
Mannheimia hemolytica in sheep
All serotypes
found in nose
little antimicrobial resistance
Mannheimia hemolytica in Bovines
Serotypes 1,2,6
not found in nose
a lot of antimicrobial resistance
role in bacterial bronchopneumonia in calves
Shipping fever is caused by
M. haemolytica and P multocida
Pathogenesis of Shipping Fever
Stress due to transport, Endogenous M. haemlytica multiplies at the nasopharynx, secondary infection by P. multocida capsule type A,D
Lesions associated with Shipping Fever/ bacterial broncho-pneumonia in calves
fibrinous necrotizing bronchopneumonia
serofibrinous pleuritis
well defined pneumonia zones
Dx of Shipping Fever
BAL from lungs and nose
Tx/Prev of Shipping Fever
Prev: Feed, decrease stomach fermentation, ventilation, thermoregulation, vx
Tx: antibiotics (evt. + cortico)
Bibersteinia trehalosi
Resembles bovine hemorrhagic septicaemia
septicaemiae at 5-12 mo
acute mortality
Haemophillis general
Host specific
2wks-4mnths (mainly at weaning)
obligate symbionts
NAD dependent
What causes Glasser dz
Haemophilus parasuis
Pathogenesis of H. parasuis?
endogenic-exogenic infection
septicimea
meninigitis
polyserositis, polyarthritis
Tx and Prev of Haemonphilus parasuis
Tx- antimicrobials
Prev- vx (not always successful)
Haemophilus felis causes
chronic pneumonia and conjunctivitis
Histophilus somnei general
difficult dx, needs 2 days incubation w/ 5-10% CO2
colonizes the urogenital mucosal surfaces of ruminants
obligate symbiotic
What causes Sleeper’s dz in ruminants?
Histophilus somnei
Histophilus somnei virulence factors
LOS
OMPs
Activation of caspases and thrombocytes
biofilms
Histophilus somnei dx and tx
bacterial culture
antibiotics
vx available
What causes Infectious Coryza in backyard chickens
Avibacterium paragallinarum
Virulence factors for Avibacterium paragallinarum
Capsule
Adhesins
Endotoxin
Iron acquisition
Pathogenesis and symptoms of Avibacterium paragallinarum
carriers, transmission via water or aerosol, stress
symptoms
-acute: general symptoms and anorexia, conjunctivitis
-subacute: sinusitis and pus, edema head and neck
can become chronic
Avibacterium paragallinarum dx, tx, and prev
dx- bacterial culture
tx- antibiotics FAST
prev- eliminate carriers
Ornithobacterium rhinotracheale
fac pathogen in different bird species
18 serotypes
relatively new, 1994
Symptoms of O. Rhinotracheale
nose exudate
facial oedema
mortality low 1-3%
Dx of O. Rhinotracheale
Slow bacterial culture, needs CO2
ELISA for A serotype
Tx and Prev for O. Rhinotracheale
tx- antibiotics, natural and acquired resistance. Need suceptability testing
prev- climate, vx
Actinobacillus pleuropneumoniae general
swine worldwide young pigs, less than 6 mo obligate symbiotic host specific not so resistant in enviroment
2 Biotypes of Actinobacillus pleuropneumoniae
1- NAD dependent
2- NAD independent
16 serotypes
Epidemiology of Actinobacillus pleuropneumoniae
spread from pig to pig, direct or aerosol
pen to pen, ventilation, regrouping of pigs, farmer
carriers: nose, tonsils, lung nodule
Virulence factors of Actinobacillus pleuroneumoniae
fimbriae
iron needed for multpilication
Apx toxins
Apx toxins in Actinobacillus
a type of RTX toxin
causes cell lysis and reduces phagocytic function
Symptoms of Actinobacillus pleuropnuemoniae
respiratory distress
general symptoms
acute- dead pig w/ blood on mouth, necrotizing pneumonia
chronic- pigs don’t grow as much