Exam Questions

Question 1

Describe how a DNA profile can be made from a small sample of DNA (6 marks)

Answer 1

• PCR/polymerase chain reaction to create multiple copies of DNA.
• Credit detail of PCR e.g. temperatures at step, use of (primers/dna polymerase/dna nucleotides).
• Reference to restriction endonucleases used to produce DNA fragments
• Reference to gel electrophoresis
• Load dna fragments into wells in the agarose gel
• Apply (electric current/charge) across the agarose gel
• Reference to the use of (southern blotting/DNA probe/radioactive labelling) for visualisation.