Exam questions Flashcards
Why are viruses acellular?
- Don’t have a cell membrane
- Not made of cells
Why are viruses non-living?
- Can’t independently respire/replicate
Describe 2 functions of golgi apparatus:
- Modifies and packages proteins
- Modifies and packages lipids
What is a proteome?
- All the range of proteins a cell can code for
Describe how quaternary protein formed from its monomers
- Amino acids joined by peptide bond(s);
- (By) condensation reaction(s);
- Secondary structure is formed by hydrogen
bonding; - Tertiary structure formed by interactions
(between R groups); - Quaternary structure contains >1 polypeptide
Describe structure of DNA
- Nucleotide= deoxyribose, phosphate and nitrogenous base
- Phosphodiester bonds between nucleotides
- Hydrogen bonding between adenine and thymine, and cytosine and guanine
- Double helix structure
- DNA is associated with histones
- DNA is a polynucleotide
Give 2 reasons for conserving rainforests:
- Medicine produced
- Conserve plants/animals
Describe how DNA is related to its function:
- Double-stranded allowing semi-conservative replication
- Base sequence and complementary base pairing allowing accurate replication
- Hydrogen bonds are weak so easily broken for replication
- Large so can store a lot of information
- Sugar-phosphate backbone provides strength
- Helix so compact
Describe ways in which all dipeptides are similar in which they might differ:
Similarities
- Consist of 2 amino acids
- Joined by peptide bond
- Contain amine group
- Contain carboxyl group
- Contain C,H,N and O
Differences
- Contain different amino acids
- Variable R group
Describe how a non-competitive inhibitor can reduce the rate of an
enzyme-controlled reaction
- Binds to the allosteric site on enzyme
- This changes the tertiary structure of the enzyme and so the active site no longer complementary
- So enzyme-substrate complexes can no longer bind
Two proteins have the same number and type of amino acids but different
tertiary structures.
Explain why:
- Different sequence of amino acids
- Results in ionic,hydrogen, disulphide bonds in different places
Describe how a phosphodiester bond is formed between two nucleotides
within a DNA molecule.
- Condensation reaction
- Between phosphate and deoxyribose
- Catalysed by DNA Polymerase
Why does the DNA need to be single stranded in DNA Replication?
- Each strand acts as a template
- Determines the order of nucleotides
Contrast the structures of ATP and a nucleotide found in DNA to give two
differences.
- ATP contains ribose whereas DNA contains deoxyribose
- ATP has 3 phosphate groups, DNA has 1
- ATP nitrogenous base is adenine whereas DNA is varied
Why can new nucleotides can only be added in a 5’ to 3’
direction.
- DNA is antiparallel
- DNA Polymerase is specific
- Can only bind to 5’ end of DNA
- Shapes of 5’ and 3’ end are different
Describe how an ATP molecule is formed from its component molecules.
- Condensation reaction
- Between adenine, ribose and three phosphate groups
- Catalysed by ATP Synthase
Explain five properties that make water important for organisms.
- Polar so acts as a metabolite for hydrolysis and condensation reactions
- Solvent so useful in metabollic reactions
- High specific heat capacity so buffers changes in temperature
- High latent heat of vaporisation so provides a cooling effect
- Cohesive so allow movement of column of water in plants
- Cohesive so produces surface tension supporting small organisms
Describe the roles of iron ions, sodium ions, and phosphate ions in cells.
- Iron= Haemoglobin transports oxygen around the body
- Sodium ions= contransport of glucose as sodium ions actively transported out of the ileum epithelial cells, lowering concentration in the cell
- Phosphate ions= joins adjacent nucleotides together, produce atp, phosphorylates other compounds, making them more reactive
What is the cell wall of plants, fungi and bacteria made of?
plants= cellulose
fungi= chitin
bacteria= murein
Why may nucleus not be visible under a microscope?
it is not stained
what is the name of the membrane of a vacuole
tonoplast
what is the name of membrane of chloroplast and the cytoplasm of chloroplast
- thylakoid membrane
- stroma
what is the name of the membrane of the mitochondria and the cytoplasm
-cristae
-mitochondrial matrix
Give one advantage of viewing a biological specimen using a transmission
electron microscope compared with using a scanning electron microscope.
- higher resolution so can see internal structures
Contrast how an optical microscope and a transmission electron
microscope work and contrast the limitations of their use when studying
cells.
- TEM uses electrons, OP uses light
- TEM has greater resolution so can see smaller organelles under microscope
- TEM can only see dead specimen, OP can view living
- TEM cant show colour, OP can
- TEM is a more complex procedure OP isnt
- TEM needs thinner specimen
- TEM focuses using magnets, OP uses glass lens
Name two structures in a eukaryotic cell that cannot be identified using an
optical microscope.
- Mitochondria
-Ribosomes
Describe how you could make a temporary mount of a piece of plant tissue
to observe the position of starch grains in the cells when using an optical
(light) microscope.
- Add a drop of water to the glass slide
- Obtain a thin slice of tissue and place flat on slide
- Stain with iodine in potassium iodide
- Gently lower coverslip with mounted needle
Describe binary fission in bacteria
- Circular DNA replicates
- Plasmid replicates
- Cytoplasm divides