Exam One Flashcards
1
Q
cyanogen bromide cleavage
A
carboxyl side of M
2
Q
trypsin cleavage
A
carboxyl side of K and R
3
Q
chymotrypsin cleavage
A
carboxyl side of Y (tryosine), W (tryptophan0, F, L, M
4
Q
carboxypeptidease A cleavage
A
AA side of C-terminal AA (not R, lysine K, or P)
5
Q
protease enzyme cleavage
A
“simplify” (unlinking disulfide bonds) problem of protein seq by cutting up protein into smaller frags
6
Q
dialysis bag
A
allows small mols to leave through small holes large protein mols stay (ex: kidneys)
7
Q
gel filtration
A
basic way to separate proteins; beads made of dextan work bc of shape w/ cavities on surface small proteins take longer, large proteins go faster
8
Q
cation exchanger
A
ion exchange works to separate AAs
