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BIOC4332: Biochemistry II > Exam I Review > Flashcards

Exam I Review Flashcards

1
Q

A cDNA library produced by PCR amplification and sequenced by synthesis in the Illumina platform would contain all these elements except:

A. The coding sequence
B. The gene promoter
C. The 5’ region
D. The exons
E. The 3’ region

A

B. The gene promoter

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2
Q

What is the product of deamination of cytosine?

A

Uracil

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3
Q

What are the proteins required for DNA double strand repair by NHEJ?

A

Ku

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4
Q

PCR can be used to amplify:

A. All of these
B. Genomic DNA
C. None of these
D. cDNA
E. Plasmid DNA

A

A. All of these

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5
Q

The alpha and beta globin genes are an example of [homologs/orthologs].

A

homologs

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6
Q

What is the mechanism that cell use to increase genetic diversity during meiosis?

A

Homologous recombination (HR)

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7
Q

What is the purpose of the two primer sequences (oligonucleotides) used in PCR amplification?

A

Hybridize to opposite strands on either side of the target sequence

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8
Q

A DNA molecule with multiple nucleotide mismatches would melt/separate at a [lower/higher] temperature than a DNA molecule without mismatches.

A

lower

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9
Q

What is the mechanism exploited by cell in the immune system to generate millions of antibodies?

A

Non-homologous end joining (NHEJ)

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10
Q

What are the type of enzymes that remove DNA supercoiling?

A

Topoisomerases

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11
Q

DNA primase is a [DNA/RNA]-dependent, [DNA/RNA] polymerase

A

DNA; RNA

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12
Q

____ Is a series of overlapping DNA sequences used to make a physical map that reconstructs the DNA sequence of a chromosome.

A

Contig

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13
Q

Does reverse transcriptase need a primer?

A

No

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14
Q

What is the function of translesion polymerase?

A

It polymerizes over damaged bases.

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15
Q

Does telomerase carry its own template?

A

Yes

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16
Q

The ____ strand is the DNA strand that has codons in their DNA form.

A

Coding strand

17
Q

What is the linear progression of nucleotides that comprise the total DNA of an organism?

A

Genome

18
Q

Proofreading exonuclease activity relies on the lack of hybridization of the most recently added nucleotide. True or false?

A

True

19
Q

What are SINEs?

A

Transposons that rely in the reverse transcriptase encoded by other transposable elements

20
Q

Is NHEJ error prone?

A

Yes

21
Q

What is the function of the Southern blot?

A

Detects complementary DNA by hybridizing labeled oligonucleotide

22
Q

What does Sanger sequencing use?

A

Nucleotides lacking the 3’ hydroxyl

23
Q

What is the function of Shelterin?

A

Protects chromosome ends

24
Q

What is the function of LINEs?

A

Autonomously encode for its own reverse transcriptase

25
Q

The ____ strand is read by RNA polymerase to make a complementary RNA strand.

A

template

26
Q

What is the function of flap endonuclease?

A

It removes primers from Okazaki fragments

27
Q

What are the enzymes that generate double strand breaks in the DNA and require ATP?

A

Topoisomerase II

28
Q

What are the enzymes that don’t require ATP and generate nicks in the DNA?

A

Topoisomerase I

29
Q

cDNA can be prepared using ___ as a template.

A

RNA

30
Q

To prepare cDNA by PCR, primer oligonucleotides and ____ are needed.

A

dNTPs

31
Q

What enzyme can generate cDNA in PCR?

A

Reverse transcriptase

32
Q

_____ are enzymes that can hydrolyze DNA in the interior of a double helix.

A

Endonucleases

33
Q

The _____ helps to make DNA polymerase more processive.

A

sliding clamp

34
Q

The polymerase chain reaction does require hybridization of nucleic acids. True or false?

A

True

35
Q

Plasmids are linear DNA molecules that can replicate autonomously. True or false?

A

False

36
Q

If you construct a complete human cDNA library it would contain approximately __% of the human genome.

A

1

37
Q

Is CGGGGC a palindromic DNA sequence?

A

No

38
Q

What do levels of DNA methylation identify (in prokaryotes)?

A

If an origin has been fired

39
Q

DNA methylation triggers origin firing in eukaryotes. True or false?

A

False

Only in prokaryotes

BIOC4332: Biochemistry II (7 decks)

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