Exam I Review

Question 1

A cDNA library produced by PCR amplification and sequenced by synthesis in the Illumina platform would contain all these elements except:

A. The coding sequence
B. The gene promoter
C. The 5’ region
D. The exons
E. The 3’ region

Answer 1

B. The gene promoter

Question 2

What is the product of deamination of cytosine?

Answer 2

Uracil

Question 3

What are the proteins required for DNA double strand repair by NHEJ?

Answer 3

Ku

Question 4

PCR can be used to amplify:

A. All of these
B. Genomic DNA
C. None of these
D. cDNA
E. Plasmid DNA

Answer 4

A. All of these

Question 5

The alpha and beta globin genes are an example of [homologs/orthologs].

Answer 5

homologs

Question 6

What is the mechanism that cell use to increase genetic diversity during meiosis?

Answer 6

Homologous recombination (HR)

Question 7

What is the purpose of the two primer sequences (oligonucleotides) used in PCR amplification?

Answer 7

Hybridize to opposite strands on either side of the target sequence

Question 8

A DNA molecule with multiple nucleotide mismatches would melt/separate at a [lower/higher] temperature than a DNA molecule without mismatches.

Answer 8

lower

Question 9

What is the mechanism exploited by cell in the immune system to generate millions of antibodies?

Answer 9

Non-homologous end joining (NHEJ)

Question 10

What are the type of enzymes that remove DNA supercoiling?

Answer 10

Topoisomerases

Question 11

DNA primase is a [DNA/RNA]-dependent, [DNA/RNA] polymerase

Answer 11

DNA; RNA

Question 12

____ Is a series of overlapping DNA sequences used to make a physical map that reconstructs the DNA sequence of a chromosome.

Answer 12

Contig

Question 13

Does reverse transcriptase need a primer?

Answer 13

No

Question 14

What is the function of translesion polymerase?

Answer 14

It polymerizes over damaged bases.

Question 15

Does telomerase carry its own template?

Answer 15

Yes

Question 16

The ____ strand is the DNA strand that has codons in their DNA form.

Answer 16

Coding strand

Question 17

What is the linear progression of nucleotides that comprise the total DNA of an organism?

Answer 17

Genome

Question 18

Proofreading exonuclease activity relies on the lack of hybridization of the most recently added nucleotide. True or false?

Answer 18

True

Question 19

What are SINEs?

Answer 19

Transposons that rely in the reverse transcriptase encoded by other transposable elements

Question 20

Is NHEJ error prone?

Answer 20

Yes

Question 21

What is the function of the Southern blot?

Answer 21

Detects complementary DNA by hybridizing labeled oligonucleotide

Question 22

What does Sanger sequencing use?

Answer 22

Nucleotides lacking the 3’ hydroxyl

Question 23

What is the function of Shelterin?

Answer 23

Protects chromosome ends

Question 24

What is the function of LINEs?

Answer 24

Autonomously encode for its own reverse transcriptase

Question 25

The ____ strand is read by RNA polymerase to make a complementary RNA strand.

Answer 25

template

Question 26

What is the function of flap endonuclease?

Answer 26

It removes primers from Okazaki fragments

Question 27

What are the enzymes that generate double strand breaks in the DNA and require ATP?

Answer 27

Topoisomerase II

Question 28

What are the enzymes that don't require ATP and generate nicks in the DNA?

Answer 28

Topoisomerase I

Question 29

cDNA can be prepared using ___ as a template.

Answer 29

RNA

Question 30

To prepare cDNA by PCR, primer oligonucleotides and ____ are needed.

Answer 30

dNTPs

Question 31

What enzyme can generate cDNA in PCR?

Answer 31

Reverse transcriptase

Question 32

_____ are enzymes that can hydrolyze DNA in the interior of a double helix.

Answer 32

Endonucleases

Question 33

The _____ helps to make DNA polymerase more processive.

Answer 33

sliding clamp

Question 34

The polymerase chain reaction does require hybridization of nucleic acids. True or false?

Answer 34

True

Question 35

Plasmids are linear DNA molecules that can replicate autonomously. True or false?

Answer 35

False

Question 36

If you construct a complete human cDNA library it would contain approximately __% of the human genome.

Answer 36

1

Question 37

Is CGGGGC a palindromic DNA sequence?

Answer 37

No

Question 38

What do levels of DNA methylation identify (in prokaryotes)?

Answer 38

If an origin has been fired

Question 39

DNA methylation triggers origin firing in eukaryotes. True or false?

Answer 39

False Only in prokaryotes