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BCH403 > Exam 6 > Flashcards

Exam 6 Flashcards

1
Q

(Exam 6) __________ is not processed prior to translation.
-Prokaryotic mRNA
-Eukaryotic tRNA
-Prokaryotic tRNA
-Eukaryotic mRNA
-Eukaryotic rRNA

A

-Prokaryotic mRNA

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2
Q

(Exam 6) When the first and second nucleotides are methylated, we refer to the 5’ cap as:
-Cap-1
-Cap-2

A

-Cap-2

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3
Q

(Exam 6)The transcriptional termination signal in eukaryotes, AAUAAA, is recognized and bound by:
-Poly A Polymerase
-Cleavage/Polyadenylation Specificity Factor
-Cleavage Factor I
-Cleavage Factor II
-Cleavage Stimulation Factor

A

-Cleavage/Polyadenylation Specificity Factor

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4
Q

(Exam 6) ________ is the first SnRNP to recognize and bind to the branch point.
-SnRNP U1
-SnRNP U2
-SnRNP U4
-SnRNP U5
-SnRNP U6

A

-SnRNP U2

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5
Q

(Exam 6) A CGG codon is mutated to AGG. This is an example of:
-Missense mutation (or non-synonymous)
-Sense mutation (or synonymous mutations)

A

-Sense mutation (or synonymous mutations)

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6
Q

(Exam 6) For this question, please refer to the follow mRNA sequence:
ACCGGAUAUUGACGCUACUG
The third codon in reading frame 3 (RF3) encodes for:
-Leucine (Leu, L)
-Isoleucine (Iso, I)
-Valine (Val, V)
-Serine (Ser, S)
-Stop codon (Termination codon)

A

-Leucine (Leu, L)

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7
Q

(Exam 6) When found in the anticodon, 5’ UGA 3’ can form Wobble interactions with (please note the direction of the anticodons and anticodon):
-5’ UCG 3’
-5’ UCA 3

A

-5’ UCG 3’

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8
Q

(Exam 6) The substrates for amino acyl-tRNA synthestases are:
-an amino acid and tRNA
-two amino acids and tRNA
-an amino acid, ATP, and tRNA
-an amino acid, GTP, and tRNA
-Amino acyl-tRNA and Peptidyl-tRNA

A

-an amino acid, ATP, and tRNA

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9
Q

(Exam 6) A frameshift mutation will result in:
-a change of one amino acid to a different amino acid.
-a change of one amino acid encoding codon to a stop codon.
-a change to an amino acid encoding codon, but no change to the encoded amino acid.
-an insertion or deletion of one or more codons.
-a change in the sequence and length in the encoded protein sequence

A

-a change in the sequence and length in the encoded protein sequence

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10
Q

(Exam 6) The Shine Dalgarno sequence is recognized by the ________ on the small ribosomal subunit.
-16S rRNA
-18S rRNA

A

-16S rRNA

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11
Q

(Exam 6) Release factor 1 recognizes and binds to a stop codon positioned in the __________ of the ribosome.
-A site
-P site

A

-A site

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12
Q

(Exam 6) __________ binds to the 5’ cap and poly A tail to circularize mRNA.
-eIF1
-eIF2
-eIF3
-eIF4F
-eIF4A

A

-eIF4F

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13
Q

(Exam 6) n eukaryotes, __________ is recharged by __________.
-eEF-2; eEF-1βγ
-EF-G; EF-Ts
-EF-Tu; EF-Ts
-eEF-1α; eEF-1βγ
-eEF-1βγ; eEF-1α

A

-eEF-1α; eEF-1βγ

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14
Q

(Exam 6) In eukaryotes, the formation of a peptide bond is catalyzed by an adenosine residue found on the ________.
-23S rRNA
-5.8S rRNA
-18S rRNA
-16S rRNA
-28S rRNA

A

-28S rRNA

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15
Q

(Exam 6) Translocation of an unfolded protein in bacteria requires ________ as an energy source.
-ATP
-Proton (H+) pump

A

-ATP

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16
Q

(Exam 6) Proteins targeted to the Golgi are expressed by ________ and transported to the Golgi via __________.
-Free ribosomes; TOM barrels
-Free ribosomes; Vesicles
-Rough endoplasmic reticulum ribosomes; TOM barrels
-Rough endoplasmic reticulum ribosomes; Vesicles
-Free ribosomes; Nuclear pores

A

-Rough endoplasmic reticulum ribosomes; Vesicles

17
Q

(Exam 6) Human microRNA (miRNA):
-is an exogenous source of RNA used in RNAi.
-is an endonuclease involved in anti-viral response.
-helps regulate gene expression by activating an RNase to degrade target mRNAs.
-form a complex with Dicer to form RNA-induced silencing complex (RISC).
-helps regulate gene expression by inhibiting translational initiation

A

-helps regulate gene expression by inhibiting translational initiation

18
Q

(Exam 6) Argonaute:
-Is a riboendonuclease (RNase) found in the nucleus.
-Selects which strand of miRNA will be used to target mRNA.
-Degrades the strand of miRNA that is not chosen to target mRNA.
-Stabilizes aptamers to form a riboswitch.
-Something about Jason and a golden fleece.

A

-Selects which strand of miRNA will be used to target mRNA.

19
Q

(Exam 6) Polymerase Chain Reaction (PCR) uses synthetic primers to replace the need for:
-DNA polymerase
-DNA Primase
-DNA Helicase
-Clamp loader
-Sliding clamp

A

-DNA Primase

20
Q

(Exam 6) Restriction enzymes recognize and cleave DNA at a:
-restriction site.
-restriction endonuclease.
-promoter.
-Shine Dalgarno sequence.
-Kozak sequence

A

-restriction site.

21
Q

(Exam 6) DNA is cut with a restriction enzyme that leaves a 5’ overhang. These overhangs are an example of:
-Blunt ends
-Sticky ends

A

-Sticky ends

22
Q

(Exam 6) A technique that amplifies and isolates a specific DNA fragment is called:
-Southern blot
-Northern blot
-Western blot
-Polymerase Chain Reaction (PCR)
-Restriction digestion

A

-Polymerase Chain Reaction (PCR)

23
Q

(Exam 6) A technique used to push proteins through pores to separate molecules only by length is called:
-Agarose gel electrophoresis
-Restriction digestion
-SDS-Polyacrylamide gel electrophoresis (SDS-PAGE)
-SDS-Agarose gel electrophoresis (SDS-AGE)
-Native Polyacrylamide gel electrophoresis (Native PAGE)

A

-SDS-Polyacrylamide gel electrophoresis (SDS-PAGE)

24
Q

(Exam 6) Western Blot:
-utilizes a complimentary probe to identify a DNA sequence of interest in a sample run on a gel.
-utilizes a complimentary probe to identify an RNA sequence of interest in a sample run on a gel.
-utilizes a complimentary probe to identify a protein sequence of interest in a sample run on a gel.
-utilizes an antibody to identify a protein sequence of interest in a sample run on a gel.
-utilizes an antibody to identify a DNA sequence of interest in a sample run on a gel.

A

-utilizes an antibody to identify a protein sequence of interest in a sample run on a gel.

25
Q

(Exam 6) __________ can be used as a high-throughput method to detect the presence of a protein of interest.
-Northern blot
-Western blot
-Enzyme-linked Immunosorbent Assay
-Immunohistochemistry
-Chromatin immunoprecipitation

A

-Enzyme-linked Immunosorbent Assay

26
Q

(Exam 6) Which one of the following techniques is used to detect proteins?
-Western blot
-Southern blot

A

-Western blot

27
Q

(Exam 6) A technique used to determine if a specific fragment of RNA is in your sample is:
-Southern blot
-Northern blot
-Western blot
-Eastern blot
-SSW Blot

A

-Northern blot

28
Q

(Exam 6) To accurately measure gene expression at the level of RNA (i.e., to determine how much RNA is present in your sample), you should use:
-qPCR
-RT-PCR
-Northern blot
-Southern blot
-a reporter construct

A

-qPCR

29
Q

(Exam 6) __________ can be used to determine the DNA-binding sequence of a protein.
-DNase I Footprinting
-Electrophoretic Mobility Shift Assay

A

-DNase I Footprinting

30
Q

(Exam 6) Chromatin Immunoprecipitation is used to study __________ in vivo.
-DNA-protein interactions for any DNA-binding protein
-DNA-histone interactions only

A

-DNA-protein interactions for any DNA-binding protein

BCH403 (6 decks)

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