Exam 4 Flashcards

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1
Q

meat quality factors

A

tenderness
juiciness
flavor
color
odor

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2
Q

3 most important factors for palatability

A

Flavor (taste and aroma) * most important
tenderness
juiciness

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3
Q

meat palatability

A

Tenderness is essential for consumer acceptance
90% of US steaks are considered tender or very tender

recent studies suggest flavor is equally important
when tenderness is within an acceptable range, then flavor becomes MORE important to eating satisfaction
58% of consumers indicate flavor has more impact than tenderness
43% of consumers indicate tenderness has more impact than juiciness

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4
Q

Is tenderness influenced by marbling?

A

only 10% of the variation in tenderness is associated with the marbling “halo effect”

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5
Q

Meat tenderness

A

tenderness is essential for consumer acceptance
tenderness is one of the few variables that add value

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6
Q

what influences tenderness

A

contractile state of the muscle: shorter sarcomere length=tougher
enzymatic degradation: calpain protease system
connective tissue:
marbling: higher marbling=more tender

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7
Q

marbling and the halo effect

A

only 10% of the variation in tenderness is explained by marbling
marbling helps to lubricate the mouth and reduce the density
at least 2% marbling is needed for acceptable tenderness

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8
Q

flavor perception

A

flavor is based on perception not sensation: combo of taste and aroma

taste: perceived by taste buds primarily on the tongue

odor/aroma: detected by the olfactory system
greatest contributor to perceived flavor
low molecular weight volatile compounds bind to olfactory receptors and are responsible for perceived flavors

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9
Q

basic tastes in meat

A

sweet
sour
bitter
salty
umami

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10
Q

odor/aroma in meat

A

raw meat has little aroma and has a blood-like flavor
however raw meat is a reservoir of precursor compounds that develop into flavor and odor-causing compounds as a result of chemical reaction that occur during cooking

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11
Q

cooked meat flavors

A

stem from:
the Maillard reaction, which combines amines and sugars to produce “meaty” flavor
thermal oxidation of lipids during cooking which produces volatiles/aromatics accounting for species flavor difference

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12
Q

volatiles produced during cooking that contributes to meat flavor

A

compounds formed in mailard reaction: aldehydes pyrazines

compounds formed during lipid thermal oxidation: alcohols aldehydes, hydrocarbons, ketones

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13
Q

amount composition of IM fat affects flavor profile of meat

A

IM lipids and associated volatiles produced during cooking are primary contributors to the flavor and aroma of meat
animal production practices that affect meat flavor do so primarily via effects on amount of composition of IM fat

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14
Q

effect of marbling degree on the palatability of a positive sensory experience

A

the higher the marbling the more likely a positive sensory experience

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15
Q

grain finishing for 100 days

A

produces grain-fed beef flavor
increase beef flavor, decrease fishy, decrease milky-oily

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16
Q

pre-harvest stress can influence flavor

A

important to avoid stress associated with handling and transport prior to harvest

compared to normal beef, dark cutting beef has a less desirable flavor with a higher incidence of off-flavors often characterized as:
bloody/serum like, sour, bitter, soapy

PSE pork tends to have an acidic flavor

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17
Q

Forage-finished beef affects on beef fat composition

A

higher levels of omega-3 polyunsaturated fatty acids, C 18:1 trans fatty acids, and stearic acid
all of which are negatively correlated with flavor desirability
flavor often characterized as grassy, gamey, livery, milky/oily, or fishy

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18
Q

Grain-finished beef affects on beef fat composition

A

higher levels of oleic acid, linoleic acid, and other monounsaturated fatty acids
all of which are positively correlated with flavor desirability
desired before fat flavor often characterized as “buttery”

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19
Q

Other factors affecting flavor

A

species: red meat and poultry
breed: bos indicus vs bos taurus
sex: androstenone, skatole
diet: grain fed vs grass fed
age: young lamb vs mutton
packaging: MAP, over-wrap, vacuum
fat
muscle
aging
enhancement
cooking method

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20
Q

Meat Quality

A

tenderness
juiciness
flavor
odor
color
multiple processes deteriorate these properties: bacterial, chemical, enzymatic, and physical activity
maintenance of these desirable properties= shelf life

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21
Q

meat discoloration

A

meat discolors much before it spoils
the best indicator of spoilage is smell

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22
Q

importance of meat color

A

consumer base purchasing decisions off od meat color: more important than any other factor
color is an indicator of freshness or wholesomeness: if not bright cherry red may be perceived as spoiled or unwholesome
discoloration influences meat purchases: at 20% discoloration discrimination by consumers, at 40% discoloration rejection by consumers
discoloration influences meat purchase: 15% of retail beef is discounted due to discoloration, and annual revenue loss >3.7 billion

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23
Q

importance of meat color continued

A

color plays a large role in determining retail case life:
consumers consider bright red/pink to be desireable, deviations may create unacceptability
consumers determine quality through appearance
discoloration is considered unwholesome

many biochemical, physical, and microbiological factors affect color: understanding causes for discoloration helps with management of color
utilization of tools to maximize retail case life

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24
Q

how is meat color measured

A

CIE L, a, and b* values

hue: trueness of red; arctangent (b/a)
chroma: intensity or degree of color saturation; (a^2 + b^2)^1/2

subjective color measurements: how the individual eye perceives color, trained or consumer panels

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25
Q

L*

A

lightness (z axis: 0-100)
greater L* = lighter sample
lower L*= darker sample

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26
Q

a*

A

redness (x-axis; +60 to - 60)
greater a* means more red

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27
Q

b*

A

yellowness (Y axis; +60 to -60)
greater b* means more yellow the sample

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28
Q

myoglobin

A

sarcoplasmic protein

primary protein responsible for meat color
contain 8 alpha helies and a prosthetic group

water soluble and contains protein and non protein portions: non protein heme ring, protein globin

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29
Q

heme ring of myoglobin

A

centrally located Fe that can for 6 bonds:
4 bonds with pyrrole nitrogen
5th bond with proximal histidine (amino acid portion of globin)
6th binding site can reversibly bind ligands and other compounds

nature of the group attached to the 6th binding site & chemical state of iron determines meat color

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30
Q

chemical state of iron

A

color influenced by compounds that interact with Fe in the Heme Ring: ability to bind with myoglobin depends on valance (charge) of Fe

when reduced (ferrous, 2+) myoglobin readily binds oxygen or H2O

the valance state of Fe depends on availability of electrons: ETC, enzymes use residual O2 long after harvest

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31
Q

ferrous vs ferric

A

ferrous= reduced
ferric= oxidized

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32
Q

chemical forms of myoglobin

A

primary forms of myoglobin:
deoxymyoglobin (DMb)
oxymyoglobin (OMb)
metmyoglobin (MMb)
carboxymyoglobin (COMb)

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33
Q

deoxymyoglobin

A

occurs when H2O or no ligand is available for binding to 6th site
heme iron is ferrous (Fe2+)
publish-red or purplish-pink color
requires very low or no oxygen: commonly seen in vacuum packages

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34
Q

oxymyoglobin

A

occcurs when O2 is bound to the 6th binding site: myoblgoin has very high affinity for O2
bloom= oxygenation

heme iron is ferrous (Fe2+): same valance state as BMb (no change in electrons
bright cherry red color
requires continuous exposure to O2: OMb penetration increases with exposure, not a stable formation, eventually promotes oxidation

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35
Q

metmyoglobin

A

oxidation of ferrous (Fe2+) Mb to ferric (Fe3+): loss of an electron

resulting in brown or tan color: undesirable

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36
Q

why does MMb form

A

insufficient oxygen to form OMb
depletion of electrons
contamination (aerobic bacteria)

MMb formation is gradual: MMb located between internal DMb and superficial Omb, gradually thickens and moves to surface

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37
Q

carboxymyoglobin uses

A

COMb is visually indistinguishable from Pmb
CO inhibits pathogenic bacteria growth
CO inhibits lipid oxidation promoting desirable flavor
CO promotes color, but doesn’t inhibit natural spoilage processes
odor of fresh meat equally important as color

38
Q

carboxymyoglobin

A

formed when DMb is exposed to CO: not a naturally occurring reaction, Myoglobin has a higher affinity for CO than O2 (CO poisoning)

Heme iron is ferrous (Fe2+), forming a stable red color (stability depends on CO availability)

CO is approved for use in meat packaging systems to 0.4%

39
Q

Metmyoglobin reduction

A

this process doesn’t last forever

reduction of MMb brown (Fe3+) to DMB red (Fe2+): changes meat color from becoming brown

a muscles ability to transition is termed metmyoglobin reducing ability: varies by muscle, postmortem age, pH, Temp, etc

40
Q

electrons are the currency of meat color

A

deoxymyoglin Fe2+ (purple) is oxidized to oxymyoglobin

oxymyoglonim Fe2+ (red) is reduced to metmyoglobin

Metmyoglobin Fe3+ (brown) can be reduced back to deoxymyoglobin as long as enzymes are available to do so

41
Q

other forms of myoglobin

A

Nitric Oxide Myoglobin (NOMb): cure pink, stable with the addition of heat

Cyanmetmyoglobin (CNMb): stable red

Suflmyoglobin (SHMb): green, product of microbial contamination

Choleglobin (H2O2): green, product of microbial contamination

42
Q

Nitric Oxide Myoglobin

A

involved in processed meat production: nitrites are added for preservation, flavor and color development (curing)

Myoglobin (purple) + NO = nitric oxide myoglobin (red) + heat = nitrosylhnechromagen (cured pink)

43
Q

Nitric Oxide Myoglobin (natural)

A

the use of celery powder and sea salt to add nitrates to cure meat

44
Q

pre harvest effects on meat color: diet

A

antioxidants prevent oxidation retarding the development of MMb:
vitamin E supplementation to live cattle improves OMb stability

45
Q

pre-harvest effects on meat color: housing and management

A

loose housed cattle have darker muscles than stalled cattle
increased # of slow twitch fibers, vascularization and oxidative metabolism

46
Q

pre-harvest effects on meat color: stress

A

PSE and DFD

47
Q

pre-harvest effects on meat color: chill rate

A

temperature at which muscle enters rigor affects color: deviations in fat thickness, thickness of muscle
non-uniformity in muscle color due to pH/temperature decline

48
Q

why is packaging important

A

food packaging serves to protect products against deteriorating effects, contain the product, communicate to the consumer as a marketing tool, and provide consumers with ease of use and convenience

many quality factors can be influenced by packaging: if meat doesn’t look good a consumer will not purchase it

49
Q

packaging properties that influence shelf life

A

package type: aerobic and anaerobic
headspace and atmosphere
packaging equipment
temperature

utilization of correct package extends shelf life
increasing shelf life by 1-2 days would save US meat industry up to $1 billion annually

50
Q

History

A

70+ years ago: butcher shops and full-service meat markets

1950s to present: convenience, increased federal oversight, and competition with other proteins lead to commercialized packaging

51
Q

Histotial changes in meat packaging

A

meat cut and harvest by the consumer: animals harvested as needed
meat cut by butcher: wrapped in wax paper
1950s: increased overwrap packaging
1950s-1990s: advances in packaging technology, barrier packaging (restrict atmosphere influence), are permeability, moisture permeability
1990s-current: implementation of new tech, case ready, modified atmosphere, skin pack, Darfresh, Nitrite embedded, etc

52
Q

change from passive to active packaging

A

active packaging is the incorporation of specific compounds into packaging systems to maintain or extend product quality and shelf life while intelligent or smart packaging provides for sensing of the food properties or package environment to inform the processor, retailer, or the consumer of the status of the environment or food

has evolved to provide and enhance many functions for the product itself:
use of antioxidants, antimicrobials, and novel package types. smart packaging tells you if product is good or bad

53
Q

wholesale meat packaging

A

relies on vacuum packaging technology: primals/subprimals are centrally packaged before distribution

critical to efficiency of modern meat packaging industries: advanced equipment multi-chambered & heat sealing
can package at minimum 50 pieces per minute

54
Q

vacuum packaging

A

utilized for “boxed” meat
oxygen impermeable bags: 3-ply laminates
ethyl vinyl acetate (tough outer layer), saran (middle oxygen layer), irradiate ethyl vinyl acetate (heat sealing)

extend shelf life:
reduce aerobic spoilage bacteria, no oxygen=less oxidation, requires strict temperature control, control of anaerobic microorganisms

disadvantages: purge, consumer acceptability (no oxygen= deoxymyoglobin= purple color)

55
Q

retail meat packaging

A

more variety than wholesale packaging
more focus on shelf-life maintenance and appearance
-traditional overwrap, chub, vacuum on a board

56
Q

traditional overwrap packaging

A

traditional: meat cut and packaged on site
uses polymer based tray and barrier films
moisture impermeable: keeps moisture out and keeps moisture in
oxygen permeable: allows for “bloomed” color (oxymyoglobin), prolonged exposure leads to oxidation
light permeable: rapid photo-oxidation, leads to faded color issues

disadvantage: leads to spoilage the fastest

57
Q

case ready packaging

A

what is case ready: fabrication nd packaging of “consumer-sized” retail items in a centralized non-retail location for shipping to retail location

why case ready: an average of 10% greater profits versus store-cut meat. more control and less cross-contact contamination, less labor for retail stores

500 mil in 1997
2.8 billion in 2005
83% of all protein sales in 2018

58
Q

modified atmosphere packaging

A

MAP: defined as removal or replacement of the atmosphere surrounding the product before sealing in vapor-barrier materials
multiple formats: tray sealed, master bag and overwrap tray, vacuum skin packaging, laminate, pouches

MAP is the method used for carboxymyoglobin color preservation in meat

59
Q

Tray sealed high oxygen:

A

80% O2 and 20% CO2 most common,
promotes the development of oxymyoglobin,
decreased shelf life via aerobic bacterial growth and oxidation

60
Q

Tray sealed low oxygen

A

various amounts of CO2 and N2
promotes development of deoxymyoglobin
more than 30% CO2 results in absorption of CO2 by meat

61
Q

tray sealed Carbon Monoxide

A

no oxygen
0.4% approved for use in meat
promotes development of carboxymyoglobin

62
Q

changes in case ready

A

ten years ago tray sealing was sweeping the industry
currently declining acceptance of tray sealed meats: bulky increasing shipping costs, consumer perception

63
Q

how can industry utilize case ready without giving appearance of case ready

A

master-bag or master package

64
Q

master packaging

A

placement of “traditional” package types into MAP packaging: gives the appearance that meat was packaged at retail store

internal package: overwrap, one exception is PVC is micro-perforated

Multiple atmospheres can be used in master packaging: most common is carbon monoxide

65
Q

vacuum skin packagin

A

relatively new type of case-ready

advantages: enhanced shelf life, reduced oxidation & microbial growth, attractive appearance, small footprint

disadvantages: formation of deoxymyoglobin, can be overcome with newer technologies (nitrate films), expensive

66
Q

rollstock, laminates, & pouches

A

relatively new type of case-ready
advantages: convenience, attractive appearance, small footprint
disadvantages: rollstock machines often have problems

67
Q

chub packaging

A

increasing in prevalnce: >15% of ground beef sold in 2016
common for ground meats

advantages: economical, branding and identity, easy to handle/store, longer shelf life

disadvantages: formation of deoxymyoglobin, temperature control is essential, consumer cannot see meat

68
Q

ground beef shelf life days

A

least long shelf life: carcass trim ground in store, case ready overwrap

longest shelf life: bulk chub reground in store, vacuum packaged primal

69
Q

ground beef shelf life days

A

least long shelf life: carcass trim ground in store, case ready overwrap

longest shelf life: bulk chub reground in store, vacuum packaged primal

70
Q

poultry packaging

A

largely different from red meats
unlike red meat, color isn’t primary in poultry packaging

packages are selected to enhance safety and convenience:
whole bird bags, SSD/SES stretch and shrink films, pillow packs, tray-sealed

71
Q

packaging trends

A

convenience is driving the market: consumers want meat that is easy to store/prepare/cook. smaller and single serve portions, cook-in-bag, recipes on the bag

sustainability and green packaging: reduced environmental impact, recyclable & biodegradable, made from reused material (plant pulp, recycled paper waste)

72
Q

Whats in a label

A

seven components to each label
1) accurate product name
2) list of ingredients
3) name and place of business packer, manufacturer, or person whom product is prepared
4) net weight
5) official federal or state inspection stamp
6) official plant number
7) handling instructions, if the product is perishable

73
Q

pathogen

A

bacteria (microorganisms) that cause infections (disease).
they can be spread through food consumption and cause foodborne illness

CDC estimates that 1/6 Americans get sicked 128,000 get hospitalized and 3,000 die of foodborne illness.

74
Q

4 important bacteria classifications

A

Gram stain and bacterial cell wall
temperature requirement
oxygen requirement
pH of Growth

75
Q

Gram Stain

A

knowing this will help us understand which antibiotics will work on the infection

76
Q

temperature requirements bacteria

A

psychrophiles: grow at 0 degrees C or below max 20 degrees C, optimal 15

facultative psychrophiles: can grow at 0 degrees C, optimal 20-30 degrees C

mesophiles: grow between 25-40 degrees C, optimal 37 degrees C, *human pathogens

thermophiles: grow above 40 degrees C

hyperthermophiles: optimum temperature above 80 degrees C

77
Q

Oxygen requirements

A

*important for packaging
obligate aerobes: require oxygen
microaerophiles: require oxygen at low concentrations
obligate anaerobes: require no oxygen
facultative anaerobes: can use oxygen prefer no oxygen
aerotolerant anaerobes: no need for oxygen but tolerant

78
Q

pH requirements

A

acidophiles:
neutrophiles
alkaliphiles

79
Q

Stages of bacterial growth

A

1) LAG bacteria are metabolically active but not growing (where we want to keep bacteria)

2) EXPONENTIAL time of bacteria growth (doubling)

3) STATIONARY growth and death rate are equal (nutrients depleted)

4) DEATH exponential decrease in number of living cells (nutrients depleted, waste accumulated)

80
Q

Intrinsic factors that affect bacterial growth

A

physical and chemical properties of the meeat
nutrients
pH
water activity

81
Q

extrinsic factors

A

associated with the environment in wich the meat is stored
temperature
gaseous environment
storage time

82
Q

Pathogens of major concern

A

Shiga toxin-producing Escherichia coli (STEC) O157:H7
Salmonella enterica
campylobacter jejuni
listeria monocytogenes

83
Q

Shiga Toxin produding E. coli

A

Enterobacteriaceae
gram negative, rod shaped, non-spore forming, motile
mesophile (35 degrees C), facultative anaerobe, neutrophile

infection dose 10 cells,
beef specifically ground beef, the 7 STEC are listed as adulterants in fresh/raw non-intact beef

84
Q

Salmonella

A

enterobacteriaceae
gram negative, rod shaped, flagellated motile
mesophile (37 degrees C), facultative anaerobes, neutrophile

infection dose 1000 cells non-typhoidal to 100,000 enteric fever cells
poultry products, pork and beef, specifically ground beef (lymph nodes)

85
Q

Campylobacter

A

campylobacteriacae
gram negative, sprial shaped, motile
thermophilic (37-42 degrees C), microaerophilic (O2 5-10%), neutrophils

infection dose <500 cells
poultry and pork products (cases increase as poultry consumption increase)
C. jejuni os responsible for more than 80% of enteric campylobacter infections and E. coli 10%

86
Q

Lister Monocytogenes

A

listeriaceae
gram positive, small rod shaped, flagellated motile
psychrotrophic (-1.5 to 45 degrees C, optimal 30-37), facultative anaerobes, neutrophils

infection dose <10,000,000 cells
ready to eat meats (deli meat)
adulterant in RTE meat

87
Q

Reducing Bacteria

A

MULTIPLE HURDLES
pre-harvest and post-harvest interventions
post harvest: physical decontamination, chemical interventions, refrigeration
further processing: drying, cooking, curing and smoking

88
Q

how do we evaluate these interventions

A

in lab evaluation: in-vitro broth study, in-vivo with meat
(use pathogenic bacteria)

in plant evaluation: validation study (use of surrogates)
surrogates: non-pathogenic microorganisms that behaves similar to the pathogen of interest (FSIS approved)

89
Q

Pseudomonas spp>

A

non-pathogenic meat spoilage bacteria

obligate aerobes, psychotropic,

responsible for putrefaction smell, spoilage levels produce hydrogen sulfide bonds to myoglobin resulting in green color

90
Q

Lactic Acid Producing Bacteria

A

non-pathogenic meat spoilage bacteria

aerotolerant anaerobes (can survive vacuum packaging)

lactobacillus is the largest genus
acid fermentation (sour off-flavor), gas production, and slime secretion