Exam 3/Practicum 1 Flashcards
Cell
Structural and functional unit of life
Plasma membrane consists of
Membrane lipids that form a flexible lipid bilayer
Lipid bilayer is made up of
75% phospholipids 5% glycolipids 20% cholesterol
Phospholipids consists of two parts
Phosphate heads: are polar (charged), so are hydrophilic (water-loving) Fatty acid tails: are nonpolar (no charge), so are hydrophobic (water-hating)
Glycolipids
Lipids with sugar groups on outer membrane surface
Cholesterol
Increases membrane stability
Membrane proteins
Allow cell communication with environment
Integral proteins
Function as transport proteins (channels and carriers), enzymes, or receptors
Peripheral proteins
Enzymes and cell-to-cell connections
Membrane protein tasks
Transport, receptors for signal transduction, attachment to the cytoskeleton and extracellular matrix, enzymatic activity, intercellular joining, cell to cell recognition

Transport

receptors for signal transduction

Attachment to the cytoskeleton and extracellular matrix

Enzymatic activity

Intercellular joining

Cell to cell recognition
Tight junction
Protective layer - Keeps out bacteria and other things that are unwanted in the cell
Desmosomes
Allows flexibility (give) and hold it together
Gap junctions
Allow for rapid communication
Diffusion
Area of high concentration to an area of lower concentration
Energy not required
Speed of diffusion
Size (small) and temperature (high)
Diffusion
The nonpolar, hydrophobic lipid core of plasma membrane blocks diffusion of most molecules
Simple diffusion
Nonpolar lipid-soluble (hydrophobic) substances diffuse directly through phospholipid bilayer
Examples: oxygen, carbon dioxide, fat-soluble vitamins
Facilitated diffusion
transported passively down their concentration gradient by:
Carrier-mediated facilitated diffusion
Channel-mediated facilitated diffusion
Substances move through water-filled channels
Osmosis
Diffusion of a solvent, such as water, across a selectively permeable membrane
Water moves by osmosis from areas of low solute (high water) concentration to high areas of solute (low water) concentration
Hydrostatic pressure
Osmotic pressure
Tonicity
Ability of a solution to change the shape or tone of cells by altering the cells’ internal water volume
Isotonic solution

Same osmolarity as inside the cell, so volume remains unchanged
Hypertonic solution

Higher osmolarity than inside cell, so water flows out of cell, resulting in cell shrinking
Shrinking is referred to as crenation
Hypotonic solution

Lower osmolarity than inside cell, so water flows into cell, resulting in cell swelling
Can lead to cell bursting, referred to as lysing
Active Transport
Requires carrier proteins (solute pumps)
Moves solutes against their concentration gradient (from low to high)
This requires energy (ATP)
Two types of active transport
Primary active transport
Required energy comes directly from ATP hydrolysis
Secondary active transport
Required energy is obtained indirectly from ionic gradients created by primary active transport
Sodium-potassium pump

Potassium in (high) - negative charge inside
Sodium out (high)
Pumps where it’s already highly concentrated
Phagocytosis
Cell eating
Pinocytosis
Cell drinking
Voltage
Difference in electrical charge between two points
Resting membrane potential (RMP)
Electrical potential energy produced by separation of oppositely charged particles across plasma membrane in all cells
Compartmentalization
Separation
Mitochondria
“Power plant” of cells because they produce most of cell’s energy molecules (ATP)
Ribosomes
Site of protein synthesis
Made up of protein and ribosomal RNA (rRNA)
Free ribosomes
Free floating
Membrane-bound ribosomes
Attached to membrane of endoplasmic reticulum (ER)
Rough ER
Site of synthesis of proteins that will be secreted from cell
Smooth ER
Lipid metabolism
Absorption, synthesis, and transport of fats
Detoxification of certain chemicals
Converting of glycogen to free glucose
Storage and release of calcium
Golgi Apparatus
Modifies, concentrates, and packages proteins and lipids received from rough ER
Peroxisomes
Detoxifying
Two main detoxifiers: oxidase and catalase
Play a role in breakdown and synthesis of fatty acids
Lysosomes
Digestive enzymes (acid hydrolases)
Centrosome
Cell center
Generates microtubules; organizes mitotic spindle (for cell division or mitosis)
Consisting of centrioles — microtubular (churros)
Cilia
Whiplike, motile extensions on surfaces of certain cells
Thousands of cilia work together in sweeping motion to move substances (example: mucus) across cell surfaces in one direction
Flagella
Longer extensions that propel the whole cell
Microvilli
fingerlike extensions of plasma membrane used to increase surface area for absorption
Chromatin
Consists of 30% threadlike strands of DNA, 60% histone proteins, and 10% RNA
Chromosomes
Condensed chromatin
Interphase
Cell grows and carries on its usual activities
Cell division
Cell splits into two
S Phase - Synthesis
Replicate DNA
DNA replication
RNA starts replication by laying down short strand that acts as a primer
DNA polymerase attaches to primer and begins adding nucleotides to form new strand
End result: two identical “daughter” DNA molecules are formed from the original
Semiconservative replication
Each new double-stranded DNA is composed of one old strand and one new strand
M Phase - Mitosis
Prophase
Metaphase
Anaphase
Telophase
Prophase
Early prophase: chromosomes become visible and centrosomes separate and migrate toward opposite poles, mitotic spindles and asters form
Late prophase: nuclear envelope breaks up
Metaphase
Chromosomes are lined up at cell’s equator
Anaphase
Chromosomes split
Telophase
Cell starts to split
Cytokinesis
Two daughter cells PINCHED apart
Nitrogen bases
Adenine, Guanine, Thymine, and Cytosine
RNA
“go-between” molecule that links DNA to proteins
RNA copies the DNA code in nucleus, then carries it into cytoplasm to ribosomes
Three types of RNA:
Messenger RNA (mRNA)
Ribosomal RNA (rRNA)
Transfer RNA (tRNA)
Messenger RNA (mRNA)
Code is copied with complementary base pairs - transcription
Ribosomal RNA (rRNA)
Structural component of ribosomes
Transfer RNA (tRNAs)
Carrier of amino acids - Translation
Transcription
DNA information coded in mRNA
Transferring code held in DNA gene base sequence to complementary base sequence of mRNA
Translation
mRNA decoded to assemble polypeptides
Step of protein synthesis where the language of nucleic acids (base sequence) is translated into the language of proteins (amino acid sequence)
Complimentary Base Pairs
A-U
G-C
Comes in triplets then turns into codons in mRNA
AUG is start codon – beginning in mRNA
UAC is start anticodon - beginning in tRNA
Tissues
Groups of cells similar in structure that perform common or related function
Histology
Study of tissues
Four basic tissue types:
epithelial, connective, muscle, and nervous tissue

Polarity
Top and bottom
Apical surface
Upper free side, is exposed to surface or cavity
Some have specialized projections called microvilli
Basal surface
Lower attached side, faces inwards toward body
Attaches to basal lamina, an adhesive sheet that holds basal surface of epithelial cells to underlying cells
Epithelial Tissues
No blood vessels, supplied by nerve vessels
Covering a surface or lines the vessel of an inner tube
high regenerative capacities
Simple epithelia
Single layer
Stratified epithelia
Two or more layers
Squamous
Flattened and scale-like
Cuboidal
Box-like, cube
Columnar
Tall, column-like
Apical layer
Top, free edge
Gland
One or more cells that makes and secretes an aqueous fluid called a secretion
Endocrine
Internally secreting (example: hormones)
Exocrine
Externally secreting (example: sweat)
Endocrine glands
Ductless glands
Unicellular exocrine glands
Mucous cells and goblet cells
Connective tissue
Connective tissue proper
Cartilage
Bone
Blood
All connective tissues have three main elements
Ground substance
Fibers
Cells
Extracellular matrix
Ground substance
Fibers
Connective tissue fibers
Collagen – thick purple
Elastic – medium red
Reticular – small blue
“Blast” cells
Immature form of cell that actively secretes ground substance and ECM fibers
Fibroblasts found in connective tissue proper
Chondroblasts found in cartilage
Osteoblasts found in bone
Hematopoietic stem cells in bone marrow
Fat cells
Store nutrients
White blood cells
Neutrophils, eosinophils, lymphocytes
Tissue response to injury
Mast cells
Initiate local inflammatory response against foreign microorganisms they detect
Macrophages
Phagocytic cells that “eat” dead cells, microorganisms; function in immune system
Four main classes of connective tissue:
Connective tissue proper
Cartilage
Bone
Blood
Covering and Lining Membranes
Composed of an epithelium bound to underlying connective tissue proper layer
Three types of membranes
Cutaneous membranes
Mucous membranes
Serous membranes
Repair can occur in two major ways:
Regeneration and Fibrosis
Regeneration
Same kind of tissue replaces destroyed tissue, so original function is restored
Fibrosis
Connective tissue replaces destroyed tissue, and original function lost






















Frontal, Median, Transverse planes


Prophase

Metaphase

Anaphase

Telophase
Four Basic Tissue Types and Basic Functions
Epithelial tissue – covering
Connective tissue – support
Muscle tissue – movement
Nervous tissue – control

Simple Squamous Epithelium
Location: alveoli of lung
Function: diffusion and filtration

Simple Cuboidal Epithelium
Location: kidney tubules
Function: secretion and absorption

Simple Columnar Ephithelium
Location: digestive tract
Function: secretion of mucous/absorption

Pseudostratified columnar epithelium
Location: trachea
Function: produces and moves mucous

Stratified squamous epithelium
Location: epidermis of skin
Function: protects from abrasion

Transitional epithelium
Location: urinary Bladder
Function: stretches

Arreolar
Location: under epithelial tissue throughout body
Function: wraps and cushions organs

Adipose (fat – lipid)
Location: fat
Function: energy storage, insulation, and protection

Dense Regular
Location: tendons
Function: connects muscle to bone

Elastic
Location: aorta
Function: recoils if stretched
Three types of cartilage:
Hyaline cartilage
Elastic cartilage
Fibrocartilage
Hyaline cartilage
Hyaline cartilage
Most abundant; “gristle”
Appears as shiny bluish glass
Found at tips of long bones, nose, trachea, larynx, and cartilage of the ribs
Elastic cartilage
Similar to hyaline but with more elastic fibers
Found in ears and epiglottis
Fibrocartilage
Properties between hyaline and dense regular tissue
Strong, so found in areas such as intervertebral discs and knee

Firbocartilage
Location: intervertebral discs
Function: shock absorber

Bones
Location: bones
Function: supports, protects, allows movement by muscle

Blood
Location: blood vessel
Function: transports gases and nutrients

Skeletal Muscle
Location: skeletal muscles
Function: voluntary movement

Cardiac Muscle
Location: heart
Function: pumps blood

Smooth Muscle
Location: hollow organs
Function: involuntary movement of substances