Exam 3 part 5

Question 1

attaches amino acids to tRNA

Answer 1

aminoacyl t-RNA synthetase

Question 2

aminoacyl-tRNA synthetase has 2 active sites

Answer 2

synthesis site,

proofreading site

Question 3

sites on ribosome

Answer 3

APE
Aminoacyl t-RNA binding site
Peptidyl-t-RNA binding site
Exit site

Question 4

what delivers aminoacyl tRNAs?

Answer 4

EF-Tu

contains a GTP, which is hydrolyzed to make a GDP

Question 5

moves tRNA from A to P site

Answer 5

EF-G

Question 6

two ways of high fidelity

Answer 6

kinetic proofreading- ribosome waits to allow wrong tRNA to come off
induced fit- small ribosome holds mRNA, conformational change occurs when correct one is added

Question 7

RNA molecules that posses catalytic activity

Answer 7

ribozymes

Question 8

added for stop codon

Answer 8

release factor

Question 9

sequence that marks the start codon in prokaryotes

Answer 9

Shine-Dalgarno sequence

AGGAGGU

Question 10

sequence that marks the start codon in eukaryotes

Answer 10

Kozak sequence

ACC-AUG-G

Question 11

eukaryotic initiation

Answer 11

eIF2 delivers the initiation meth to P site of small ribosome
eIF4E and eIF4G are cap binding proteins
when AUG is found, eIF2 is hydrolyzed
large ribosome joins small ribosome