Exam 3 part 4

Question 1

attaches G residue to 5’ end of mRNA

Answer 1

guanylyltransferase

Question 2

location of guanylyltransferase

Answer 2

attached to CTD

Question 3

after guanylyltransferase detaches

Answer 3

Cap-Binding Complex (CBC) attaches to 5’ cap,

binds mRNA to CTD

Question 4

before termination point of mRNA,

what binds to it

Answer 4

AAUAAA,

CPSF

Question 5

at termination point of mRNA,

what binds to it

Answer 5

CA

CstF

Question 6

average poly A tail length

Answer 6

250

Question 7

after termination point of mRNA

Answer 7

GU-rich region,

degraded away in nucleus

Question 8

creates poly A tail

Answer 8

Poly-A Polymerase (PAP)

Question 9

attaches to poly A tail

Answer 9

Poly-A Binding Protein

Question 10

splicing is carried out by

Answer 10

spliceosome

Question 11

spliceosome is made of

Answer 11

5 snRNPs or snurps, and other proteins

Question 12

snRNPs (snurps) are made of

Answer 12

snRNAs and proteins

U1, U2, U4, U5, U6

Question 13

names of snurps

Answer 13

U1, U2, U4-U6, U5

Question 14

explain the process in splicing

Answer 14

U1 attaches to 5' splice junction
BBP and U2AF attach to adenine
U2 replaces BBP and U2AF
U4-U6 and U5 "triple snRNP" come in and bring U1 and U2 together
U6 replaces U1
U1 and U4 release
splice is complete

Question 15

U2, U5 and U6 make the

Answer 15

catalytic core